دراسة وظيفية و جزيئية لبعض المورثات الايضية للنساء المصابات بسرطان الثدي والخاضعات للعلاج بعقار التاموكسيفين

Physiological and molecular study of some metabolic gene of breast cancer women Subjected to treatment with tamoxifen

Breast cancer is frequently a hormonally dependent cancer especially estrogen , therefore, this study was designed For the purpose of studying  the role of the drug tamoxifen and its impact on this hormone in addition to studying the genotype of some metabolic genes responsible for a metabolism of tamoxifen in women in Iraq.

This study involved 100 women with breast cancer and undergoing treatment (tamoxifen), and 30 healthy women apparently.

The two groups of women ages ranged from 20-80 years old collected blood samples for the purpose of measuring some internal hormones such as estrogen, testosterone , prolactin hormone, liver enzymes, other blood parameters , and the genotype of CYP2D6, CYP3A4 , CYP3A5 genes  .

The results showed that the highest percentage of breast cancer for women in the age group (41-60) and by 58% in addition to the largest number of women patients and the healthy group begins menstruation to have aged (10-13 years) and 68% of patients and 63.33 % of the healthy . The results showed that 88% of patients are married and the healthy group 53.3% and the 51.13% of the patients had marriageable age category 10-20 years.

The study also found that 32% of the patients group who have the family history of breast cancer, while 68% of those who do not have a family history.

The results of this study showed a significant decrease in estrogen levels after it has been removed the tumor and receiving radiation and analogue LHRH and finally a drug tamoxifen The hormones prolactin and testosterone did not show any significant differences between the patient group and healthy as well as liver enzymes, albumin , globulin and fibrinogen The total cholesterol and low cholesterol density LDL may have shown significantly higher in the patients group And significant decrease in high-density cholesterol HDL..

Study the genotype of the gene CYP2D6 * 4 also showed that most of the samples did not digest was due to the lack of the presence of this allele The samples digested was mostly homozygous  (qq) increased slightly to 45%, which is accountable for the production of the enzyme metabolic weak effectiveness of Metabolic Drug tamoxifen (OR = 1) it was also noted there was not significant differences between the HEALTHY group and the group of patients the gene CYP3A4 * 1B digest for most of the samples did not happen either samples digested were mostly homogeneous type heterozygous (pp) wild-type 77.7% OR = 1.43, and the gene CYP3A5 * 3 digested samples were mostly homogeneous type heterozygous (pp) wild-type (63%), followed by the differential type, with no significant differences between the species.

We conclude from this study that the drug tamoxifen effect lowering of estrogen, which is the main cause of breast cancer in the cases studied in addition to not digest most of the samples of the gene CYP2D6 and that due to the possibility of lack of allele * 4, or a failure occurs in cutting and women who carry the genotype mutant (qq) that responsible  for the production of an enzyme effectiveness is weak and therefore metabolize poor property shall have to change and to find more effective drug.