دورالاصابات البكتيرية في امراض المناعة الذاتية

Role of Bacterial Infection in Autoimmune Disease

Abstract

Systematic autoimmune lupus erythematosus (SLE) is disease that impacts several tissue and organs leads to damage of these tissues.The present study has been conducted in Karbala province. A total of (60) blood and urine samples, (40) from patients women and (20) from control women were taken from women aged (20-59) years. This patient`s group divided into two groups, women suffer from urinary tract infection and the second group without infection. To determine the effect of bacterial infection on the level of (CD4+Tcell, CD19+B cell, HLA-DR and HLA-DQ) in blood using flowcytometry technique and identify the level of(interleukine-10 and interleukine-23) in serum using ELISA technique, molecular method using polymerase chain reaction technique to determine csgA presence in bacterial samples have been used.
The present study has recorded (15) infection with different types of bacteria that infect urinary tract in patients with SLE, the identification of bacterial using biochemical test and confirmed using viteck system.
The results showed significant increase in the level of CD4+Tcells in patients that have systematic lupus erythematosus (P ≤ 0.0001) and compared to in SLE patients with infection than without getting an infection as well as control, the results also showed significant increase in the level of CD19+B cells in SLE patients compared to control, and significant increase in the level of CD19+B cells in patients that have infection (csgA positive) (P ≤ 0.05) relative to patients without infection.

Furthermore, the result showed significant decrease in the level of HLA-DR in patients compared to control (P ≤ 0.01) and also in patients with infection compared to control (P ≤ 0.05). While the level of HLA-DQ showed decrease in patients compared to control and increase in patient with infection compared to patients without infection but the result was not significant.
Finally the results of the study demonstrated that there was significant increase in concentration of proinflammatory cytokine IL-23 in Compared between patients to control(P ≤ 0.001) And also significant increase in patients with infection relative to control(P ≤ 0.0001), while results of the concentration of anti-inflammatory cytokine IL-10 showed no differences in Compared with monitoring, all groups of patients.
The current study concluded that the bacterial infections in SLE patients that cause UTI especially bacteria that express csgA gene activate the adaptive immune response especially T and B cells.