دور الابيكواينين-10 والكيسببتين-10 في التغيرات التكاثرية لذكور الجرذ خلال مرحلة ماقبل البلوغ الجنسي

The role of ubiquinone-10 and kisspeptin-10 on reproductive changes of male rat during prepuberty

Summary
This study is carried out to investigate the effects of pulsatile Kisspeptin administration for12 days on reproductive efficacy in immature male rats, and to assess the protective role of ubiquinone 10. Thirty-six male rats divided into four groups each group have 9 animals were treated as follows; control group receives dimethyl sulfoxide (DMSO) intraperitoneally, twice daily ; kisspeptin group treated with pulsatile kisspeptin (65μg/kg B.W) intraperitoneally, twice daily ; ubiquinone group treated with ubiquinone10 (10 mg/kg B.W ) orally via gavage, twice daily and kisspeptin and ubiquinone group treated with pulsatile kisspeptin (65μg/kg B.W) intraperitoneally, Combination with ubiquinone10(10 mg/kg B.W) orally via gavage, twice daily. all treatments were lasted for 12 days. animals were reared till the day 60 of age then Body weight and anogenital distance were measured,
Blood samples were taken via cardiac puncture to assess (Follicle stimulation hormone, luteinizing hormone, Testosterone, and inhibin B), and then animals were sacrificed under anesthesia to dissected & collected (testes and prostate) for measured the testicular measurements, sperm analysis and histological study.
The result revealed a significant decrease (p ≤ 0.05) in the levels of follicle-stimulating hormone, luteinizing hormone and testosterone in male rats treated with kisspeptin compared to the other groups, while the level of Inhibin B showed a significant increase (p≤0.05) in male rats treated with kisspeptin compared to animals in other groups. Coadministration of kisspeptin with ubiquinone leads to enhancing these reproductive hormones (follicle-stimulating hormone, luteinizing hormone, Testosterone, Inhibin B).
The result of body weight and testis measurements showed that there was no significant effect (p ≤ 0.05) on body weight in male rats treated with kisspeptin, while there was a significant decrease in testis weight, Gonadosomatic index, testis volume and Anogenital distance in male rats treated with kisspeptin compared to animals in control, kisspeptin, ubiquinone and kisspeptin plus ubiquinone groups. Coadministration of kisspeptin with ubiquinone leads to a significant enhancement in testicular measurements (Testis weight, Gonadosomatic index, Testis volume and Anogenital distance).
The result of sperms analysis showed that total spermatid heads, elongated spermatid and daily sperm production have a significant decrease (p ≤0.05) in the kisspeptin group compare with other groups, Coadministration of kisspeptin with ubiquinone leads to a significant improvement in the total sperm heads, elongated spermatid and daily sperm production.
The histological study of the testis of animals treated with kisspeptin in comparison with control animals shows degeneration of seminiferous tubule (sloughing and disruption of germinal epithelium, increased intraepithelial gaps, atrophied epithelial cells and placed irregularly on the basement membrane) and accumulation of pinkish edematous fluid between the seminiferous tubules, vascular engorgement and there are a little number of spermatogonia, while prostate of animals treated with kisspeptin in comparison with control animals shows degeneration of epithelial cells and sloughing of some epithelia, hyperplasia of glandular epithelial cells and of papillary projections toward the alveolar lumen .also, show some prostatic glands appear empty from the prostatic secretion. coadministration of kisspeptin with ubiquinone leads to an improvement in testicular and prostate tissue.
However, when using ubiquinone alone we noted all studied parameters near close to the normal states.
In conclusion, from our result, we conclude that kisspeptin leads to harmful effects on reproductive hormones, testicular measurements, sperm analysis and architecture of testicular and prostate when injected in the prepubertal stage. Coadministration of ubiquinone with kisspeptin leads to improvements all these studied parameters.