دور الحامض النووي للمايتوكوندريا DNA , أيون الكروم و علامات كيموحيوية في النوع الثاني لداء السكري مع و بدون أمراض القلب الإقفارية

Role of Mitochondrial DNA, Chromium Ion and biochemical parameters in Pathogenesis of Type 2 Diabetes Mellitus with and without Ischemic Heart Diseases

Abstract

Diabetes mellitus (DM) is a metabolic disorder characterized by the presence of chronic hyperglycemia accompanied by greater or lesser impairment in the metabolism of carbohydrates, lipids and proteins.
One of important complications of DM is ischemic heart disease, therefore this cross-sectional study attempt to study the correlation and associations by many variant biomarkers and the specific mutation compared to patients without ischemic heart disease. The study select two study groups, first group include type 2 diabetes mellitus type 2 with ischemic heart disease (50 patient), and the second group of the presented by type 2 diabetes mellitus without ischemic heart disease (50 patients ).
By using EDTA tube the blood sample was withdrawn from each patient, then mitDNA was extracted by specified kit. Then the extracted mitDNA from sera of type 2 diabetes mellitus with and without ischemic heart diseases was determine by using real time PCR technique to investigate specified mutation at base pair (3243) that occur in mitDNA in order to differentiate between the different diseases.
Our result found a strong positive correlation between HOIR and Insulin (r= 0.926) with significant differences (P<0.05) in the blood of patient have ischemic heart disease and the result found mid correlation (0.568) with significant differences (P < 0.05) between insulin levels with chromium in blood of patient have ischemic heart disease. When the chromium level is less than 4.25 ppb, the individual is classified as having cardiovascular disease. The element chromium has a potential association with ischemic heart disease and has been designated as a prediction marker.
The amplification of the MTLL1 gene gives one genotypes as indicated by (422bp) bands for those with homozygous wild type (AA), homozygous mutant (GG) genotypes and two genotypes bands (422 bp) for those with hetrozygouse (GA ) .
Genotype frequencies of A3243G polymorphism were found to be consistent with Hardy–Weinberg equilibrium. Allele frequencies (32 %, 58 %, 10 %) of AA, GA, GG in cases of T2DM with ischemic heart disease group and (76 %, 22 %, 2 %) of AA, GA, GG in cases of DM without ischemic heart disease group.
The level of chromium is 12.4460 ppb in sera of ischemic heart disease as compared with that in sera without ischemic heart diseases 4.99 ppb and is significant correlated (P< 0.05).