دور الحركيات الخلوية في امراضية داء المقوسات الكوندية لدى النساء المجهضات في محافظة كربلاء

The role of cytokines in the pathogenesis of Toxoplasmosis among aborted women in Karbala province

The current study was designed to detect and investigate infection with the parasite Toxoplasma gondii by detecting specific IgG and IgM antibodies in women who aborted with Toxoplasma disease, as well as studying three cytokines (IL-4, IL-8, and IL-27) and one chemokines (MCP-1). Detection of changes in the level of cellular motility in aborted women with toxoplasmosis compared to a control group and women aborted for other reasons.
A collected 117 samples of venous blood from aborted women attending some government hospitals and private medical clinics in some areas of Karbala Governorate from the period 1/11/2022 until 1/5/2023, and all the required information was recorded, after which a rapid test was conducted for all samples, and it turned out that 43 samples were Positive, of which 42 samples were positive for IgG, at a rate of 97.7%, while one sample was positive for IgM, at a rate of 2.3%. As for the results of the ELISA test for the IgG antibody, they showed that 52 out of 117 samples were positive, at a rate of 98.1%, and for the IgM antibody, one sample was positive. A rate of 1.9% and 64 negative samples, a rate of 33.7%.
Immune indicators were evaluated using ELISA technology for all 117 study samples as well as 30 control samples from non-aborted women in Al-Furat Medical Laboratory, and the results were statistically analyzed using the IBM SPSS STATISTICS program and according to the t-test at a significance level less than or equal to 0.05 and 0.01.
The results of the study showed that the incidence was high in the age group of 18-26 years, reaching 56.60%, compared to the age group of 27-36 years, which was 32.08%, while the lowest rate was 11.32% in the age group of 37- 47 years.
As for the nature of housing, the incidence of toxoplasmosis was higher in city areas by 60.38% compared to rural areas, which was 39%. 62.
As for infection rates during the study months, they varied, with the highest infection rate during the month of January at 37.74%, and the lowest rate was in April at 5.66%.
The study also showed that infection rates were higher in aborted women who raised animals, at a rate of 25% (79%). It is less than 20% in aborted women who do not raise animals. 75%. Also, the highest infection rate was in uneducated aborted women, reaching 62.26%, and lower than that in educated aborted women, at 37.74%. As for the standard of living, the highest percentage was within the good standard of living, as it was recorded in52.83% infection rate. The lowest infection rate was within the excellent standard of living, with a rate of 18.87%.
The study recorded an increase in the average serum level of cytokine IL-4, which amounted to 90.51 pg/ml in the serum sample of patients who aborted due to toxoplasmosis, while its amount in the serum sample of patients who aborted for other reasons was 69.37 pg/ml, compared to what was recorded in the sample of control women, which amounted to 21.31 pg/ml.
Cytokine IL-8 also showed a significant increase in the serum level in a sample of women aborted with toxoplasmosis, reaching 913.30 pg/ml, as well as in patients aborted for other reasons, reaching 394.21 pg/ml, compared to a control sample of 226.06 pg/ml.
As for the average serum level of cytokinetic IL-27, there was a significantly significant increase in the serum level in both the sample of women aborted with toxoplasmosis (645.96 pg/ml) and those aborted for other reasons (496.81 pg/ml) compared to the control sample (88.26 pg/ml). .
The chemokine of MCP-1 recorded high levels in a sample of patients who miscarried due to toxoplasmosis, as its average level was 272.20 pg/ml. This rate was close to what was observed in a sample of patients who miscarried for other reasons (220.97 pg/ml) compared to a sample of control women (48.83). Pg/ml) and significant positive correlations appeared between the three cytokines (IL-4, IL-8, IL-27, and chemokine (MCP-1)) under a probability level less than or equal to 0.05 and 0.01 using the Pearson correlation coefficient with a value limited to 0-1