دور حبة البركة والكركمين في مواجهة سمية الإجهاد التأكسدي الناجم عن البيسفينول أ في الجهاز التناسلي لذكور الجرذان

The Role of Nigella sativa and Curcumin vs Oxidative Stress Toxicity induced by Bisphenol A in male rats’ reproductive

Summary

Bisphenol A (BPA) could be considered as one of the most studied endocrine disruptors. BPA is an endocrine disruptor and an environmental pollutant; it can produce free radicals, which cause damage in various tissues including the tissues of reproductive system. The male reproductive system plays a crucial role in influencing the overall health as well as well-being of males, in recent years, there has been a growing prevalence of male reproductive health issues, leading to heightened concerns, various potential substances and factors are being scrutinized for their possible impact on male reproductive health at all.
Nigella sativa oil (NS) oil, had been demonstrated to help as s scavenger of free radicals and function as an antioxidant with a variety of male reproductive diseases. Besides, Curcumin (Cur.) has also been demonstrated to treat several sorts of male reproductive problems in experimental animals, hence increasing fertility; as well as, to scavenge free radicals and function as an antioxidant.
This study aimed to investigate the protective role of NS oil and Cur. against the toxic effect of BPA in male rats’ reproductive system by estimation the levels of the antioxidant enzyme Superoxide Dismutase (SOD) and Malondialdehyde(MDA), the level of sexual hormones such as (follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone), and lastly, the level of cytokines as (Interleukin6(IL-6) and tumor necrosis factor Alpha (TNF-α)). Furthermore, detect if there are any histological changes before and after treatment with this herb.
48 adult male rats, with weights of about (200-350 grams) and ages between (eight -10 weeks). These male rats were divided into eight groups six rats in each group. This study was conducted from November/2023 – March/2024.
The effect of NS oil and Cur. as protective agents against BPA on the oxidative stress parameters, showed that The effect of NS oil and Cur. as protective medicinal plants against BPA, on the oxidative stress parameters showed that, NS oil and Cur. had no significant values in MDA compared with the control group but in BPA group there was a significant increase in MDA ( P value=0.0001), in the same time there were increase in the concentration of both SOD (P value=0.0003), and catalase (P value<0.0001) significantly, with decrease in BPA group.
Moreover, the results revealed that, there were significant increase in FSH (P value=0.0001), LH (P value=0.0003) and testosterone (P value<0.0001) in the groups of BPA+NS oil, BPA+CUR. and BPA+ NS oil+ CUR , as well as, decrease in the levels of these hormones in BPA group comparing with the control.
The effect of NS oil, Cur. and the combination of NS oil+ Cur. on the cytokines found that, there were a significant decrease of IL-6 and TNF-α, (P value= 0.0001) and (P value=0.02) respectively in groups NS oil+ BPA, Cur.+ BPA and the combination of NS oil+ Cur+ BPA comparing with BPA group and control group. Besides, histopathological study of cross sections of testis and epididymis showed that the BPA-treated group showed detrimental effect of this chemicals on the testes and epididymis, while administration of NS oil and Cur. keeping the normal features of the testicular and epididymal section as comparing to the BPAtreated group. The group treated with combination of NS oil and Cur. had no synergistic effect when compared with groups treated with NS oil or Cur. alone. The study concluded that, NS oil and Cur. had enhanced protective effects against BPAinduced toxicity male reproductive system in rats. While the administration of NS oil and Cur. had no synergistic or antagonistic effect on healing the adverse effect of BPA.