علاقة التعدد الشكلي لجين MTHFR rs1801133 C/T مع مستويات Homocystine وVitamin B12 في مرضى البهاق

Relationship of the MTHFR gene rs1801133 C/T polymorphism with homocystine and vitamin B12 levels in vitiligo patients

The study was conducted to illuminate on some epidemiological variables of Vitiligo and its prevalence in Karbala Governorate and to find moral differences between races, age groups, chronic diseases, smoking and family history.. The study included 60 persons with Vitiligo and 40 healthy persons. Samples were collected from Karbala Governorate from beauty centers. Al-Kafeel Specialized Hospital and Imam Al-Hussain Teaching Hospital and separate areas that included rural and urban areas during the period (October 3, 2020 to January 27, 2021). The genetic variant of the MTHFR gene (rs1801133 C/T) was studied by PCR-ARMS technique, and the results were as follows:
The results of the epidemiological study showed that there were no significant differences between males and females in terms of rates of injuries, while the age groups showed a significant increase in the age group between 16-25 years by 33.34% compared to the rest of the groups, while the infection rate was high in the married and estimated 85% compared to the unmarried 15% and the family history of the disease and its pre-existence constituted a significant increase by 95% compared to non-existence 5%, and the place of infection was recorded on the body on the whole body and diffusely above it and by 45% compared with the rest of the places of the trunk, upper limbs, face and lower extremities which were recorded (13,9,6 , 5%), respectively. As for smokers, they recorded a significant increase by 81.8% compared to non-smokers 18.3%. As for chronic diseases, their absence recorded a significant increase by 73.4% compared with the presence of chronic diseases by 26.6%.
The blood picture of the affected patients compared to the healthy ones recorded some significant differences, as the group of patients recorded hemoglobin (1.59 ± 12.85) g/dL compared to the healthy group of 1.54 ± 14.20 g/dL, and the red blood cells of the patients were 0.56 ± 4.69 million cells/microliter of blood compared to The healthy subjects were (0.56 ± 5.17) million cells/microliter of blood, and the white blood cells recorded a significant difference of (2.41 ±6.32) million cells/microliter of blood in comparison with the healthy ones (1.64 ± 7.98) million cells/microliter of blood, while the (neutrophils, cells Lymphocytes, monocytes, eosinophils, and basophils) showed a significant difference between those infected with (13.62 ± 48.97%), (13.24 ± 41.21%), (1.65 ± 5.00%), (2.40 ± 3.55%) and (1.76 ± 1.46%), respectively. Significant difference compared to healthy controls (11.04 ± 59.07%), (8.18 ± 30.15%), (2.33 ± 6.73%), (2.00 ± 2.31%) and (0.23 ± 0.79%), respectively; As for the platelets, a significant increase was recorded in the infected group, with 138.73 ± 347.04 thousand / microliter of blood, compared with the healthy group, which recorded 55.71 ± 263.89 thousand / microliter of blood. The mean erythrocyte volume (MCV) and mean corpuscular hemoglobin (MCH) did not record any significant differences between Injured and healthy group.
The study recorded with regard to (HCT, RDW and mean platelet volume (MPV) significant differences between the injured group and the healthy and the patients scored (5.05 ± 40.64 /ml blood) (3.48 ± 12.89 µm) (1.16 ± 5.60 µm) respectively in comparison. With the group of healthy people, which were recorded (4.48 ± 44.16 /ml blood) (0.75 ± 11.48 µm) (0.83 ± 6.92 µm) respectively.
As for the homocystine amino acid variable, it did not register significant differences, while vitamin B12 recorded a significant relationship, which decreased significantly in the patient group 31.10 ± 67.93 ng/L compared to the healthy group 57.09 ± 292.10 ng/L.
As for the relationships between gender and blood picture variables, there were no significant differences between males and females for the variable hemoglobin, red blood cells, white blood cells, eggs, neutrophils, lymphocytes, monocytes, basal cells, platelets, mean platelet volume (MCV), mean corpuscular hemoglobin (MCH), leukocytes, and homocystine. Complete blood count RCDW and mean platelet volume (MPV) significantly increased in males 13.82 ± 0.73 and 5.25 ± 0.21 µm respectively compared to 11.66 ± 0.25 and 6.06 ± 0.17 µm in females, respectively.
Whereas the relationship of residence location on the blood variables of vitiligo patients recorded no statistically significant relationships for hemoglobin, red blood cells, neutrophils, lymphocytes, monocytes, basal cells, platelets, mean erythrocyte volume (MCV), mean corpuscular hemoglobin (MCH), hemocytes, complete blood count (RCDW), mean platelet volume (MPV) and Homocystine, while white blood cells recorded a statistically significant relationship to the variable white blood cells among city residents 0.41 ± 7.13 million cells/microliter compared to rural residents 5.66 ± 0.42 million cells/microliter as well as eosinophils in the city 4.08 ± 0.44 compared to rural 2.87 ± 0.38 .
As for the relationship of some blood image variables with gene frequencies, as hemoglobin, red blood cells, neutrophils, lymphocytes, monocytes, eosinophils, basophils, platelets, MCH, Hematocrite, RCDW and MPV did not record any statistically significant relationships, while the white blood cell variable recorded statistically significant relationships between 2.51 gene types. ± 6.32, 2.65 ± 5.64, 1.62 ± 7.39 million cells/microliter, and the mean value of MCV was 5.51 ± 86.02, 3.29 ± 28.78, 7.85 ± 82.98, while Homocystine recorded 4.89 ± 15.14, 4.89 ± 15.14 and 10.19 ± 20.75 L frequency. Alleles (TC, CC, TT) respectively, and a significant relationship was recorded with vitamin B12 with gene frequencies (TT, CC, TC) between healthy and infected patients.
While the relationship of vitamin B12 with gene frequencies recorded a significant increase between the frequencies of the infection group and the CT genotype recorded 57.79 ± 266.40 ng/l compared with the healthy group 3.44 ± 48.05 ng/l, while the CC genotype was recorded in the infected group 102.28 ± 337.9 ng/l. L compared to healthy subjects 4.16 ± 56.11 ng/L and the TT genotype scored 82.09 ± 291.74 ng/L compared to 5.31 ± 130.38 ng/L in healthy subjects. As for the analysis of the level of Homocystine with the gene frequency, the gene with the genotype (CC) showed the highest significant level in the amount of Homocystine in the patients, which was (18.96 ± 1.94) compared with the patients with the genotype (CT) and the genotype (TT), where their results were recorded (± 2.3114) .63) and (±2.0415.81 ng/L), respectively.
The analysis of the level of vitamin B12 with gene frequency showed the genotype (CC) gene on the There was a significant level of vitamin B12 in their blood (5 ± 12.128 ng/l) compared with patients with genotype (TC) and genotype (TT) where their results were recorded (3 ± 5.52) and (4 ± 2.56 ng/l). Straight.