قياس مستوى الاديبوكينات والحامض النووي الرايبوزي_590 وبعض المعايير الفسلجية لدى النساء البدينات المصابات بقصور الغدة الدرقية

Evaluation level of Adipokines, MicroRNA-590 and some physiological parameters in hypothyroidism obese women

Summary
Hypothyroidism is linked to elevated dyslipidemia, traditionally attributed to decreased thyroid hormone (TH). However, recent research suggests thyroid-stimulating hormone (TSH) independently influences lipid metabolism. On the other hand, miRNAs are small, single-stranded, non-coding molecules of RNA, which regulate gene expression at post-transcriptional level. The dysregulation of the expression of several miRNAs affects pathways involved in thyroid disorders pathogenesis.
This aims of this study were accomplished by the following objectives:To determine the levels of miRNA 590 in thyroid diseases. Also to assess the sensitivity and specificity of Preselection miRNA and its diagnostic utility in these circumstances. Then find the relationship between miRNA590 and certain physiological markers, such as Obestatin and Ghrelin.
A case-control study included 90 samples of women, 60 samples of women patients with thyroid disorders and 30 samples of healthy women. The demographic aspects of the patients were collected through technique (questionnaire). The patients were subjected to a complete clinical history, clinical examination, and laboratory tests, and the type of disorder was determined based on Evaluation of laboratory measurements for thyroid hormones. The enzyme-linked immunosorbent assay system (ELISA) was performed using the Competitive-ELISA principle to measure the concentrations of hormones ghrelin and obestatin in serum samples.
Statistical analysis was performed and the efficiency of the predicting value was assessed using the receiver operating characteristic (ROC).
The results indicated that serum hormone ghrelin concentrations was higher (35.26 ng /ml) in obese women of hypothyroidism, as compared with control subjects (26.92 ng/ml), on the other hand obese women of hypothyroidism have higher obestatin levels (10.99 ng/ml) compared to the control group (7.22 ng/ml). In this study, the cycle threshold (Ct) of the microRNA590 was measured using a quantitative determination present in a sample.
A lower Ct value indicates a higher abundance of target microRNA. In this case, the patients group has a higher Ct value (29.94) compared to the control group (22.14), suggesting lower levels of microRNA590 in the hypothyroidism group. Folding change, which refers to the difference in gene expression between two conditions, was indicated to be upregulation, and higher in the patient group (13.46) compared to the control group (0.95). The results show that higher TSH levels are associated with lower microRNA590 levels.
ROC curve and Area under curve (AUC) analysis for the folding change of miRNA-590 among obese women patients with hypothyroidism compared to control groups were performed. The study concluded that The miRNA-590 folding change provides high predictive value for diagnosing hypothyroidism patients, according to the ROC curve study