Heat Shock Protein 90B1 (HSP 90B1) تقييم بروتينات , B-cell Lymphoma 2 (BCL-2) و Bcl-2 Associated X Protein (Bax) وعلاقتها مع إمراضية متلازمة تكيس المبايض

Rp- Evaluation of Heat Shock Protein 90B1 (HSP 90B1), B-cell Lymphoma 2 (BCL-2) and Bcl-2 Associated X Protein (Bax) and it is relation.pdf

Polycystic ovary syndrome (PCOS) is one of the most common chronic endocrinopathies affecting between 5 and 10% of reproductive age women. Hirsutism, acne, abnormal menstrual cycle, or obesity are common presenting features in the perimenarcheal stage. PCOS is a multifactorial disorder where several factors have been linked to the development of PCOS, which could lead to female infertility due to failure of follicle maturation, embryo implantation. And combination of genetic, environmental, and endocrine factors, which are the main causes of female ovulatory dysfunction.
The pathogenesis of PCOS has long been controversial. However, evidence accrued over the past 30 years indicates that the immediate pathophysiologic abnormality underlying the vast majority of PCOS is functional ovarian hyperandrogenism and that the insulin-resistant hyperinsulinism found in half of PCOS aggravates it. One of the most common risk factors for the progress of PCOS include family history of PCOS.
Granulosa cells (GCs) play important role in oocyte maturation, fertilization, and subsequent implantation. Many researches revealed a key role of HSP90B1 and Bcl-2/Bax in the syndrome pathogenesis. Increased levels of Hsp90B1 and Bcl-2 which are anti-apoptotic proteins, and decreased levels of Bax which is pro-apoptotic protein, might be involved in the pathogenesis of PCOS.
HSP90B1 is a stress-inducible chaperone protein. Its expression levels significantly affect cell proliferation and survival, cell cycle progression and apoptosis. While the Bcl-2 protein family are the central gatekeepers of the intrinsic or mitochondrial apoptotic response , And Bax was the first Bcl-2 homologue gene to be identified acting as an apoptosis executer.
Subjects enrolled of the present study were categorized into two groups patients with PCOS and controls. Total 66 patients and 64 controls participant who were attending in Karbala gynecological and obstetric teaching hospital in Karbala, Iraq, were carried out for the period from September 2021 to June 2022. Laboratory tests were done by serological techniques sandwich ELISA for patients, were tested for specific serum human HSP90B1, Bcl-2 and Bax.
The result of the study revealed that HSP90B1 and Bax were significant (P<0.05), while Bcl-2 was showed a non-significant (P>0.05). The conclusion HSP90B1 and Bax could be candidate as a biomarkers to evaluate the pathogenesis of PCOS.