The Faculty of Veterinary Medicine at the University of Kerbala has organized a training course: “Immunohistochemistry” which has been delivered by Eng. Dr. Raed Abdul-Mahdi Qassem and Prof. Fateh Odeh Kadhem in the Physiology Laboratory.
The Dean of the Faculty, Dr. Weefaq Al-Bazi, says that this technique is usually used for the morphological characterization of tumors or other malignant tumors of tissues, and IHC antibodies are used to detect and analyze protein expression while preserving the composition, cellular properties and the original tissue structure. Chemical fixation, often with a neutral stock, Formalin (NBF), or formaldehyde, blocks the molecular interactions within and between cells, tissue samples it can be infused in paraffin wax or frozen in a cooled preservation solution before being cut into thin strips. And installed on slides for analysis. Tissue collection, preservation, and fixation can vary greatly depending on sample or target of interest.
IHC determines the presence and pattern of expression of proteins in a biological sample through a specific antibody
Link. The precise binding that occurs between the antibody and the target protein epitope enables detection
A high-quality amino acid sequence within a protein. Antibodies can also detect post-translation (PTM) modifications of the protein. Phosphorous antibodies have been used to identify specific components
Signaling pathways and studying changes in phosphorylation events in different biological contexts. Specific antibodies, to other recently developed PTMs, allowing researchers to monitor changes in acetylation, methylation, or protein prevalence status.