التأثير الوقائي لمستخلص لحاء نبات القرفة Cinnamonum zeylanicum المائي البارد ضد التهاب المفاصل الرثوي المستحث في ذكور الجرذان البيض وثأثيرها على بعض المعايير الكيموحيوية و النسجية

Protective effect of Cinnamonum zeylanicum cold water bark extract against induced rheumatoid arthritis in white male rats and its effect on some biochemical and histological parameters

Abstract
The current study aimed to know the protective effect of the aqueous extract of Cinnamon zeylanicum to reduce the effect of arthritis induced by Complete Freunds Adjuvant in white male rats by studying some changes in blood, biochemical and histological parameters, in addition to right foot thickness and body weight in experimental animals. The study was conducted in the Graduate Studies Laboratory – College of Education – Department of Life Sciences, University of Karbala from September 22 to January 15
white male rats were used and divided randomly into eight groups (5 animals for each group). The first group G1 was the negative control group orally with plain water only for twenty days and the second group G2 was injected into the sole of the right foot with a dose of 0.1 ml of Complete Freunds Adjuvant to induce rheumatoid arthritis Promised positive control group
As for the third, fourth and fifth groups, they were injected with CFA into the sole of the right foot on the first day of the experiment and after 10 days of inducing inflammation, they were orally dosed with aqueous extract of cinnamon at a concentration (250, 350 and 450 mg/kg) of body weight, respectively, daily for 20 days. As for the sixth groups G6, seventh G7 and eighth G8, they were dosed orally with aqueous extract of cinnamon only at concentrations (250, 350 and 450 mg/kg) of body weight, respectively, daily for 20 days
Blood samples were collected from the eight groups at the end of the experiment to measure the following parameters measuring the level of liver enzymes (ALP, AST, ALT and GGT) and changes in the level of lipids (TG), TC, LDL and HDL) the concentration of oxidative enzymes (MDA, GSH and (CAT) with measuring changes in the level of ( CRP) and (RF) in addition to measuring body weight and right foot thickness before the induction of arthritis and on the last day of the experiment, and the experiment showed the following results
The results of the current study showed that the development of arthritis in rats led to a significant increase in the level of liver enzymes ALP, AST, ALT and GGT and fat level Which included TG, TC and LDL in the positive control group G2 compared with the negative control group G1, while when the groups G3, G4 and G5 were dosed orally with aqueous extract of cinnamon led to a significant decrease in the level of liver enzymes and the level of lipids compared with the positive control group G2
It also caused a significant increase in the level of MDA, CRP and RF in the positive control group G2 compared with the negative control group G1, while those titrations decreased when the animals were orally dosed with aqueous extract of cinnamon in the groups G3, G4 and G5 compared with the positive control group G2, While a significant decrease in the level of HDL, GSH and CAT was observed in the positive control group G2 compared with the negative control group G1, and those titrations increased in the groups G3, G4 and G5 when compared with the positive control group G2
Rheumatoid arthritis also caused a significant increase in the thickness of the right foot in the positive control group G2 compared to the negative control group G1, and treatment with aqueous extract of cinnamon for 20 days led to a decrease in the thickness of the right foot in the treated groups compared with the positive control group G2. In addition, there was a clear decrease in body weight in the positive control group compared to the negative control group, accompanied by an increase in weight in the protective groups compared to the positive control
The results of the current study also showed that there was no negative effect of the cold aqueous extract of cinnamon on the physiological, biochemical and histological parameters studied in groups G6, G7, and G8 that dosed the extract alone.
Histological examination of the ankle joint revealed clear changes in the G2 group when compared with the G1 group, and that these changes included the enlargement of the synovial membranes and the infiltration of inflammatory cells in the area of the injury.While an improvement in those parameters was observed in the protective groups when compared with the negative control group, as the thickness of the feet decreased, accompanied by a decrease in the infiltration of inflammatory cells in the injury area.
The results of the current study showed that giving the aqueous extract of the cinnamon plant contributed to reducing the pathological effects of arthritis by stopping the damage that may occur to the affected joint, which leads to the breakdown of cartilage tissue in inflammation. Toxic damage to the body in the studied doses.