التحري الإحيائي عن التأثير المطّفر لبعض مبيدات الآفات المستعملة في مكافحة آفات الخضر

Bioassay Investigate about mutagenic effect of some pesticide that used in resistance vegetable pests

This study has been conducted to detect the mutagenic effect of this pesticides residues in tomatos and  cucumber crops .
The commercial brond and the residues  bifenthrin EC 10 % ,  propamocarbHCL SP 10% and abamectin  EC 1.8 % were given the priority in screening due to thin abundant use in vegetables crops in Kerbala province .
The plate incorporation  and Fluctuation tests using Salmonell typhim urium TA100 and TA98   , Were inplend in the screening procedures no mouse liver enzymes (S9) was used at this stage .The Results of the commercial pesticides propamocarb HCL  and bifenthrin showed that there is a mutagenic effect in the bacterial cells for all used concentrations in both strains by using the two methods . While abamectin pesticide showed no significant effect .Concerning dissipation of the three pesticides in the tomatos and cucumber crops , the results generally show that the residues of pesticides in cucumber are more than in the tomato crop for the three pesticides .When the plate incorporation method was used to investigate the influence of the pesticicdes residue in the mentioned crops , the results showed that the residue of propamocarb and bifenthrin pesticides in tomato and cucumber crops have mutagenic influence at all the levels of production pick by using TA100 strain . But when the TA98 strain was used to perform the test , no mutagenic influence was conducted after 10 and 20 days of treating the tomato plant . And when the cucumber plant was treated , the mutagenic influence has not been noticed after 20 days of the treatment .In the fluctuation test , concentrations of the residues of the propamocarbHCL and bifenthrin has shown the mutagenic influence in TA100 strain in all of the production pick of tomato and cucumber crops .The same result has been shown in TA98 strain except in the last final stage of the two production pick , which has not shown any mutagenic influence in that bacterial strain . The researcher has also noticed that the stress of the mutagenic influence in the bacterial cells is also connected  relationship with pesticide , its residues showed no mutagenic influence in both strains TA98 and TA100 by using the two methods .
The mentioned results assert the importance of using the rapid tests to investigate the material with genotoxicity in the environment specially those which mix with the food or drink of human in order to avoid them as in the case of proving that the abamectin pesticide has no mutagenic influence compared with bifenthrin and propamocarb which can be replaced by another pesticide in order to avoid the health damages might be resulted from . It is too early to judge these two pesticides because the used method shold be correlated other live systems like using tissue culture cell or Eucaryotic cell like Yeasts . Any way the growth of the large numbers of chemical material  intermingle in the food series of human , direct us to use cheap and rapid ways for the initial evaluation which highly assists testing the materials that are considered dangerous on the human health .