التخليق الاخضر لمركبات السلينيوم واوكسيد الزنك النانوية من الكركمين وتقييم فعاليتها التثبيطية على بكتريا Pseudomonas aeruginosa و Staphylococcus aureus

Green synthesis of selenium-zinc oxide nano composites from curcumin and evaluation of their inhibitory activity on Staphylococcus aureus and Pseudomonas aeruginosa

Abstract

The current study included testing the antibacterial effect of curcumin (Curcumin) and the two nanocomplexes (Curcumin/zinc oxide Nps) (Curcumin/selenium Nps) on two types of bacteria, one of them positive for Gram stain Staphylococcus aureus isolated from urine, nose and wounds, and the other negative for Gram stain of Pseudomonas aeruginosa. Burn injuries and surgical wounds, and by 50 isolates for each bacterial type, which were isolated from several hospitals in Babylon Governorate (Musayyib General Hospital, Al-Turky Hospital and Al-Jamhory Hospital) and diagnosed by phenotypic and biochemical methods by the researcher, and the work was carried out during the period between 10/18/2020 and 1/24 /2021. As well as the use of two types of antibiotics: Gentamycin and Imipenem for Gram-negative bacteria and Vancomycin and Ciprofloxacin for Gram-positive bacteria. The two nanocomposites (Curcumin/zinc oxide Nps) and (Curcumin/selenium Nps) were prepared by green synthesis, and these compounds were diagnosed by several methods, which is, UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR). ), X-Ray Diffraction Microscope (XRD), Scanning Electron Microscope (SEM). The MIC test was carried out by dilution in liquid medium, and it was found that the minimum inhibitory concentration of curcumin is 250 and 500 µg/mL for Staphylococcus aureus and Pseudomonas aeruginosa , respectively. As for Cur/zno NPs, it gave 1000 and 2000 µg/mL for each of Staphylococcus aureus and Pseudomonas aeruginosa , respectively. While the MIC of cur/se NPs was 125 and 250 µg/mL for Staphylococcus aureus and Pseudomonas aeruginosa , respectively. Then the inhibitory activity of nanocomposites and free curcumin was evaluated in addition to several types of antibiotics (Ciprofloxacin, Imipenem, Gentamycin Vancomycin) by the the disc diffusion test, and it was highly effective against the types of the studied bacteria. The results showed that gram-positive Staphylococcus aureus was more sensitive to nanocomposites and antibiotics than gram-negative Pseudomonas aeruginosa The results also showed an increase in the effect of nanocomposites on both types of bacteria compared with free curcumin, and this increase resulted from the synergistic action between the two components of the nanocomposite. The two nanocompounds (Curcumin/selenium Nps and Curcumin/zinc oxide Nps) are alternatives to the antibiotics imipenem and Gentamicin, respectively, due to their affinity for their in vitro inhibitory effects on Pseudomonas aeruginosa The two nanocompounds (Curcumin/selenium Nps and Curcumin/zinc oxide Nps) are alternatives to Ciprofloxacin and Vancomycin, respectively, due to their affinity for their in vitro inhibitory effects on Staphylococcus aureus