المعالجة الاحيائية لسم الافلاتوكسين B1 ومبيد bifenthrin باستخدام بعض انواع البكتريا

Rp. Bioremediation for Aflatoxin B1 and bifenthrin pesticide by Using Some Species of Bacteria pdf.

This study aimed to evaluate the effectiveness of some species of bacterial isolated from dairy in the treatment of toxic aflatoxin B1 and the possibility of appointment one isolate them as us drug countary poison aflatoxin B1 In Invivo as well as on the assessment of the efficiency of the two species of bacteria, Pseudomonas fluorescens and Bacillus subtilis in degradation pesticide Bifenthrin and the possibility of products formula from this bacteria bioremediation of contaminated soil this pesticide .
Used Biochemical tests to determined the activity of these bacterial species Bioremediation of aflatoxin B1 and pesticide Bifenthrin.
The study results showed the effectiveness of the bacteria Lactococcus lactis in reducing the toxicity of aflatoxin externally (Invitro) since disappeared shine spot poison aflatoxin B1 treatment with bacteria from the plate Thin layer chromatography when exposed UV , reinforced this result vital tests that took place inside the body of animals albino Rat and the transaction of Aflatoxin pre- treatment bacterium Lactococcus lactis that they were all organs of the studied included the liver and kidneys, intestines and spleen completely intact while showed pathogenic changes clear in those animals treated with the organs of aflatoxin B1 untreated bacteria. It also proved of the formula manufactured from bacteria L. lactis and debilitating heat highly effective in reducing the toxicity of aflatoxin B1 demonstrated by the survival of all of the numbers of platelets and the percentage of lymphocytes within normal limits in the blood of animals treated first by formula manufactured followed by treatment of the aflatoxin B1 as it stood at 548.3 × 103 plate / ml and 59.9 %, respectively, while the numbers of platelets in the blood of animals treated of aflatoxin B1 only 1045 × 103 plate / mL and the percentage for the numbers of lymphocytes 32.7 % . Also that the number of monocyts were within normal limits in the blood of animals treated by formula followed poison aflatoxin B1 , amounting to 0.04 × 103 cells / mm3 , while bypass prepared in the blood of animals treated name of aflatoxin only natural boundaries much until it reached 1.36 × 310 cells / mm 3 on the other hand it was the preparation of vital important role in defense the tissues of animals treated by followed by treating them aflatoxin B1 , as results showed histological examination of sections of textile for organs of the liver , kidneys, intestines and spleen safety of any pathological changes , in the time it appeared pathological changes severe in all those organs of the animals organs tissues treated by aflatoxin B1 only was the emergence of necrosis and cells death of the tubules renal in kidneys either in the liver appeared to congestion and vascular dilation with multiplicity in the gaps necrotic, and intestines are other damaged as occurred lysis completely villi with cell necrosis (necrotic cellular ) and did not escape the spleen from the effects aflatoxin B1 as it appeared vascular congestion and cellular necrosis
Also of this study showed of the formula manufactured from bacteria L. lactis safety biomaterial did not have any negative effects on critical systems for Albion rat animals and the striking that this is of the Preparatory increased level of concentration of hemoglobin in the blood of animals treated them as it reached its quantity 14.6 g /100 ml , while were in the control treatment ( not treated by the Preparatory or poison aflatoxin B1) 12.2 g / 100 ml .
And demonstrated this study on the effectiveness of the bacteria Pseudomonas fluorescens in reducing the toxicity of the pesticide Bifenthrin and demonstrated by the lack of any decrease in hemoglobin levels and prepare the red blood cells in the blood of animals treated with pesticide chemical processor in advance , the bacterium P. fluorescens from their levels in the control treatment ( animals not treated with pesticide ) as of full treatment in the first 12.23 g / 100 ml and 7 × 610 balls / mm3 , respectively, while their levels in the control treatment 11.7 g / 100 ml and 6.4 × 610 balls / mm3 , respectively, while the effect of pesticide negatively in the amount of hemoglobin ( 10.93 g / 100 ml) and the preparation of red blood cells (9.4 × 610 balls / mm3) . also maintained the numbers of platelets and the percentage of lymphocytes to normal levels in the blood of animals treated with pesticide chemical that bioremedy by bacteria as it reached the 557.5 × 310 plate / ml and 51.4 % and did not differ significantly from the control treatment while snowball the numbers of platelets in the blood of animals treated by Bifenthrin only to 772.5 × 310 plate / mL which is much higher than for natural boundaries as the preparation of lymphocytes decreased to 38.6% .
The results of the histological study are consistent with the results of the study physiological to confirm the effectiveness of the bacterium P. fluorescens in the treatment of the pesticide chemical studied as results showed histological examination the absence of any pathological changes in the tissues of the organs of the liver , kidney, intestines and spleen of animals treated with pesticide pretreated bacteria . While affected negatively , those organs of the animal insecticide treatment Bifenthrin that non bioremediation as it showed the results of a microscopic examination of the clips histological presence of pathological changes histologic in the kidney was the occurrence of congestion and vascular atrophy and the disappearance of the glomerulus and inflated wall in addition to the occurrence of cell necrosis of the glomerulus and renal tubule , either in liver appeared to congestion and vascular and hemorrhage) with cell necrosis , and in the intestines Event decomposition of the villi with cell necrosis , either changes in the spleen vascular and necrosis cell in addition to hemorrhage . were the results of the therapeutic efficacy of the bacterium Bacillus subtilis relatively less for the bacteria P. fluorescens the results of the test activity degrading pesticide Bifenthrin in terms of germination of seeds of wheat to compliment the test results is vital using laboratory animals (male albino rat ) as shown bacteria P. fluorescens highly effective in reducing the toxicity of the pesticide chemical and represent the ability of grains of wheat insecticide that bioremedy by these bacteria on germination by 90 %, while the rate was 0% in the treatment of chemical pesticide untreated bacteria , while the bacteria Bacillus subtilis was less effective than the bacteria P. fluorescens
And proved of the Preparatory vital manufacturer of bacteria P. fluorescens highly effective in the treatment of the pesticide chemical Bifenthrin in the soil as the rate of germination of grains of wheat treatment of the preparation is vital 79.1 % when grown in soil contaminated with pesticide and a concentration of 1 % after ten days of agriculture , while the percentage of germination of the same grain and planted in soil contaminated with pesticide and same concentration above and the treatment of the preparation for the vital bacteria B. subtilis 50.6% as severalty is vital preparation for the two species of bacteria susceptibility on storage for 6 months .