المعالجه الحيويه للتربه الملوثه بالبسفينول أ باستخدام انواع مختلفه من البكتريا

Bioremediation of Bisphenol A Using Different Types of Bacteria

Summary

Bisphenol A (BPA), also known as 2,2-bis(hydroxyphenyl) propane, is a synthetic substance extensively employed in the manufacturing of plastics, specifically polycarbonate and epoxy resins; over numerous years, BPA has been extensively distributed into soils, sediments, as well as ground and surface waters. This study aimed to gain a greater insight into BPA removal by studying the biodegradation ability of polluted soil’s bacteria toward BPA.
In this study fifty samples were taken from polluted soils with plastic wastes of different sites of Holy Karbala province from (Center, Al-Hussainia and Aoun) regions and Babylon province from (Center and The Nile) regions; ten samples were taken from each region.
Minimal salt media (MSM) were used to identify the ability of these isolates to degrade BPA by using HPLC technique. After inoculation the bacterial isolates to the MSM with BPA as sole carbon source. The results obtained revealed that, only 31 isolates showed the ability to grow in this media
The results found that, there was no significant differences among the number of bacteria with BPA-biodegradability isolated from the regiones. In spite of that, the urban regiones (the center of Kerbala and Babylon) had a greater number of isolates with BPA-degradation compared with rural regiones (Al-Hussainia, Aoun, and Nile).
The results found that, the bacterial isolates could degrade the BPA in different ranges started from 18.7% to 99.9%. And there were significant differences of the ability of bacteria to remediate BPA such as Serratia plymuthica, Pantoea spp, Shingomonas paucimobilis, Pseudomonas aeruginosa and Bacillus spp., comparing with other isolates of the recent study such as, Acinetobacter haemolyticus, Acinetobacter lwoffii, Escherichia coli and Proteus spp., according to the BPA biodegradation capacity.
After DNA purification, bisdAB operon were identified in BPA-degrading bacteria, and the results found that this gene found in the most isolates under this study. Some bacterial isolates yielded negative results for the bisdAB gene, indicating a potential absence of this specific metabolic pathway for BPA degradation in those bacteria; this observation suggested the possibility of an alternative BPA metabolic pathway existing in these bacterial strains.
Ten of bacterial isolates were subjected to identification by using sequencing for 16rRNA gene, and the results gave more precise identification for these bacterial isolates.
We conclude that, many bacterial isolates could convert the toxic organic compound Bisphenol A to other metabolite in the media-containing it as only carbon source. And according to recent study we could employee this activity to eliminate these materials safely from the polluted soils and water efficiently in comprainal with chemicals that might be toxic to environment, human being and animals. And finally, the operon of bisAB gene detected in most isolates and this gene suggested to involved in BPA degradation.