بناء منظومة بلازما الضغط الجوي الباردة لاستخدامها في معالجة فطريات الجلد

Construction of Cold Atmospheric Pressure Plasma system for Using in Skin Fungal Treatment

Abstract

Fungal diseases affect human safety greatly. Some types of fungi show resistance to antibiotics when treated, and others require a long period of treatment. One of the modern and safe techniques is the use of cold plasma in biomedicine. The plasma jet system was constructed and used to treat dermatophytes, which included three common types: Trichophyton rubrum, Candida albicans, and Candida Tropical.
Atmospheric pressure plasma jet systems (APPJs) are designed with simple, low-cost components. A high alternating voltage generator was used, and the system consisted of a double-loop electrodes configuration, a Pyrex tube as an insulating barrier, and pure argon gas to feed the system.
The electrical properties of the system were diagnosed and the effect of gas flow rate and applied voltage on the length of the plasma jet and the temperature of the plasma was studied. Use optical emission spectroscopy to calculate electron temperature and electron density. The electron temperature was (10826.6 K) and the electron density was (1.57×1013 cm-3). The results showed that the system is suitable for use in biomedical applications.
A pure isolate of fungi was obtained on solid SDA agar. Fungal cells were treated with cold plasma at 25 °C. Treatment intervals were (2, 4, 6, 8, 10, 15, 20, 25) minutes. The results showed that the growth of the fungal cells decreases with the increase in the treatment period and thus leads to the killing of the fungal cell. In the treatment of Trichophyton rubrum fungi, the cell lost its ability to grow after 25 minutes of plasma treatment, but the time required to kill cells in the case of Candida albicans and Candida Troprum was shorter (15, 20) minutes, respectively. Cold plasma technology is considered a promising and safe technique that has succeeded in eliminating the fungi under investigation.