تأثير المستخلص الايثانولي لجذور نبات عرق السوس في مستوى انزيمات الكبد وبعض المعايير النسجية والهرمونية والوراثية لذكور الجرذ الأبيض المعاملة بعقار المايتومايسين- سي

Effect of ethanol extract of root Glycyrrhiza glabra plant in liver enzymes levels and some histological and hormonical and Genetic standards in males white rats Induced Mitomycin-C

   The study was conducted in the  Animal house of biology Department in the College of Education Science Pure – University of Karbala for the period from Decemper 2013 and until the month of August 2014 to know some of liver enzymes levels and some hormonical standard, histological tissue in addition to the cytogenetic resulted from alcoholic extract of Glycyrrhizaglabra roots on white male rats dealt with mitomycin – c drug. The study was conducted in the animal house of biology department in the collage of education science pure – university of karbala , has been the use of male rats number 75 male and randomly divided into five aggregates comprising (15 animals per group) the first group is negative control group is positive control group and it dosage oraly by mitomycin – c with 2mg/kg of body weghit for months , the third group dosage animals by alcoholic extract of Glycyrrhiza glabra roots with 500 mg/kg of body weghit before the mutayen with two weeks and for months , the fourth group dosage oraly with alcoholic extract of gly roots with 500 mg/kg of weghit body with mutagen for month , the fifth group dosage oraly with alcoholic extract of gly roots 500 mg/kg of weyhit body after the mutagen with two weeks and for months , and then with drawing the blood from these rats with above mention pathological effects occurred in the standard of this study , then measured hormones levels is testeroneharmone (Te) , triiodo thy ronineharmone (T3) , thyroxiuharmone (T4) , luteinizing harmone (LH) theresultsshows decreas (P< 0.05) in(T3,T4,LH) without any effect in (T) hormone for to positive control compared with negative control while the liver’s enzymes , Aspartate – aminotransferase (AST) , Alanine aminotransferase (ALT) , Alkaline phosphate (ALP) the results shows increase (P˂ 0.05) in both enzymes AST , ALP while shows decrease (P< 0.05) in the ALT enzymes of positive control compared with negative control , while his to pathological changes to liver tissue includes occurred congestion in the blood vessles with necrosis in hepatocytes and infiltration in the infamentary cells also includes his to logical effect in the kindey occurred bleeding in the interstitial tissue to cortex and medulla regions dealt with occurs necrosis in the renal glomeruli , while dealt with cytogenetic of mitotic index (MI) ,  chromosomal abberations (CA) in the cells of bone marrow and sperm abnormality (SA) the results shows decrease (P˂ 0.05) in the MI at rate 1.02 compared with negative control 3.4 also led treatment with mutagen increase (P˂0.05) in all CA and SA atrate (35.13 , 620.26) respectively comparision with negative control (3.62 , 154.78) while when the interaction between 500 mg/kg of plant extract and 2mg/kg of MMC was making in three forms of treatment of extract (before , with , after) mutagen MMC to assess extract activity in decrease drngaction in the standards studied , the results of hormones shows decrease (P< 0.05) in T , T4,LH hormones without any effect inT3 when treatment with extract (after) mutagen while led to increase (P˂ 0.05) in T3without any effect inT4,T,LH when treatment by the extract (with) the mutagen, while treatment (before) the mutagen recorded decrease (P˂ 0,05) in T4 and increase(P˂0.05)without any effect inT3,T while the Liver enzymes shows decrease (P< 0.05) in AST enzyme also result recorded decrease (P˂ 0.05) in ALT,ALP enzymes in treatment(after,with)mutagen without any effect in ALT,ALP in treatment(before)mutagen compare with positive control  . while the results of cytogenetic study of MI mice which treated with the extract (with , after) the mutagen performed a best result in the raising of median MI to 6.98 , 24.56 respectively compared with the positive control 1.02 at asignificant differences while the extract (before) the mutagen leds to raising the rate of MI to 4.44 compared with positive control . the result of chromosomal abbearations of mice bone marraw cells lower rate of abnormalties of treatment (before , with , after) the mutagen to (23.36 , 18.09 , 32.05) at asignificant differences compared with positive control 35.83 as atreatment with extract (after) abest result in lower of median abnormalties , the results of sperms abnormalities shows lower in the rate of abnormalities to (576.78 , 458.30 , 508.31) when treated with extract (before , with , after) the mutagen at asignificant differences compared with positive control 620.29 as treatment the extract (with) the mutagen a best in lower of median abnormalities of sperms . finally the test of changing in pathological tissue , the results of microscopically examination of liver and kidney tissues mice which treated with the extract (before,after) the mutagen a good improvement in both tissues.