تأثير مستخلص التوت البري على بعض المعايير الكيموحيوية والنسجية للكلية في الجرذان المعاملة بفلوريد الصوديوم

The Effect of Cranberry extract on some biochemical and histological parameters of the kidney in the Albino Rat treated with sodium fluoride

Abstract

The present study included the biological activity of cranberry extract on changes in some biochemical and antioxidant parameters, in addition to the study of kidney histological changes in white rats exposed to sodium fluoride renal toxicity at a dose of (20) mg / kg body weight. Oral doses for (30)days Respectively. While the other groups were trated with sodium fluoride with the same dosage added to the extract of cranberry with three doses of (75,150,225) mg / kg body weight for one month, after the end of the experiment periodthe animals were sacrificed and draw blood from the heart immediately after animal anesthesia by ketamine and studied the criteria that included (Body weight, sodium concentration, calcium concentration, potassium concentration, urea concentration, creatine concentration, total protein concentration, albumin concentration, globulin concentration) as well as antioxidants (SOD, CAT). The results of the present study showed a significant variation in the studied parameters. There was a significant increase in the concentration of (sodium, potassium, calcium, creatine, urea) while there is a significant decrease was observed in the concentration of (total protein, albumin, globulin, body weight) in addition to (SOD, CAT) in sodium fluoridetreated group when compared to control group. Histopathological examination of kidney of male rats treated with sodium fluoride at does (20 mg / kg) of body weight for 30 days, respectively showed histological changes marked by severe hemorrhage in renal tissue with apparent atrophy of some renal glomeruli and dissection of endometrial tubules causing the expansion of diameter along with the occurrence of degeneration in the cells lining the tubules compared to the control group. The results of the present study showed that the treatment with Cranberry extract in a dose of (75,150,225)mg/kg body weight and sodium fluoride at a dose of (20) mg / kg to test the effectiveness of the extract in reducing the toxic effect, sodium fluoride on the studied parameters, . A significant decrease was observed in ( sodium concentration, calcium concentration, potassium concentration, urea, creatine) and significant increase in concentration (total protein, albumin, clobulin, SOD, CAT,body weight). At the level of significance (P≤0.05) when comparing the fluoride group with the cranberry extract groups where these results led to the return of these parameters closer to the normal state in the control group, were the result showed a clear improvement in histological tests for kidney male albino rats after treatment with sodium fluoride. Glomeruli were observed in their normal size along with low blood bleeding and lack of degeneration in renal tubule cells. There were no significant differences between the concentrations of the extract. Concentrations of improvement is evident in the studied parameters biochemical and histologic. A cranberry dose of 150 mg/kg of body weight is regarded as the best dosage that led to results that were so close to those of negative control group. It was obvious from the results of current study,that cranberry extract is of a very high biological activity in resisting the effect to toxic substances for which the body of living creature is exposed to.