تحضير وتشخيص ليكاندي آزو- شف متعددة السن مشتقة من أمينات أروماتية وبعض معقداتها الكليتية مع الدراسة البيولوجية لها

Synthesis and characterization of poly dentate azo-schiff derived from aromatic amines and some of their chelate complexes with biological study of them

The study included the preparation of two new organic ligands of the azo-Schiff base type, namely
L1 = N-(2-(((E)-3-((E)-(4-ethoxyphenyl)diazenyl)benzylidene)amino)ethyl)-1-(3-((E)-(4-ethoxyphenyl)diazenyl)phenyl)methanimine
L2= (1E)-N-(2-(((E)-3-((4-phenoxyphenyl)diazenyl)benzylidene)amino)ethyl)-1-(3-((Z)-(4-phenoxyphenyl)diazenyl)phenyl)methanimine
The first ligand (L1) of the azo-Schiff base type was prepared in two steps, the first step included azotization the primary amine (4-Ethoxy aniline) and its reaction with benzaldehyde to prepare azo compound A.
The second step included condensation reaction of the compound (A) resulting from the first step with aliphatic amine (Ethylene di amine) with the addition of two drops of glacial acetic acid to form the new final azo-Schiff ligand.
The second ligand (L2) of the azo-Schiff base type was prepared in two steps, the first step included azotization of the primary amine (4-Phenoxy aniline) and its reaction with benzaldehyde to prepare azo compound B.
The second step included condensation of the compound resulting from the first step with aliphatic amine (Ethylene di amine) with the addition of two drops of glacial acetic acid to form the new final azo-Schiff ligand.
Then five complexes of each of the ligands L1 and L2 were prepared with the metal ions Fe(II), Co(II), Ni(II), Cu(II), and Zn(II). The molar ratio calculations showed that the (metal-ligand) ratio was (1:1) for all solid metal complexes.
The two newly prepared ligands were characterized by several measurements, including FT-IR, UV-Vis, 1HNMR, mass spectra, melting points, and elemental analysis (C.H.N.). All the prepared complexes were characterized by the above techniques, except for NMR and mass spectra. The magnetic susceptibility and molar conductivity of the complexes dissolved in absolute ethanol at room temperature and at a concentration of (10-3×1) were studied. The solubility of both prepared ligands and their complexes in some polar organic solvents (distilled water, ethanol, ether, dimethyl sulfoxide) was also tested.
Based on the above results, the geometric shapes of the complexes prepared in this study were proposed, and they were all octahedral. The final stage of the research included studying the biological activity of the prepared azo-Schiff base ligands and their metal complexes for two types of bacteria, one of which is Gram positive, namely Staphylococcus aureus and the other is Gram negative, namely Escherichia coli at two concentrations (250 and 500 ppm, for each of the prepared ligands and their solid complexes under study. A distinct inhibitory activity was shown for ligands and some of their complexes, which reduce the growth of these two types of bacteria.