تحضير وتشخيص مضاد نانوي هجين من الليفوفلوكساسين وتقييم كفائته التثبيطية ضد البكتريا المعزولة من قرحة القدم السكري

Preparation and Characterization of Nanohybrid Antibiotic from Levofloxacin and Evaluation its Inhibitory Efficiency against Bacteria Isolated from Diabetic Foot Ulcer

   This study included the isolation and diagnosis of the most important species of  bacteria responsible for the infection of diabetic foot ulcer . It also included the preparation and detection of hybrid nano compound , from the antibiotic levofloxacin .The inhibitory activity of the nano antibiotic was assessed against the isolated bacteria in this study also , the combination activity of the hybrid nano levofloxacin with free antibiotics Tiecoplanin and Cefixime was identified and the results showed the following :

As a total 43 samples were taken from diabetic foot ulcer patients hospitalized in Imam Al-Hussein Medical City in Sacred Kerbala Provience, It had been obtained 10 isolates (26.3) % were Proteus mirabilis, , 9 isolates (23.7) % were Staphylococcus aureus, 4 isolates (10.5) % were Escherichia coli, 4 isolates (10.5) % was obtained from Pseudomonas  spp. , 3 of them were Pseudomonas flouresence  and one was Pseudomonas aeruginosa and two isolates (5.3) % of Enterococcus faecalis , Raoultella terrigena and one isolate (2.6) % of Enterobacter cloacae , Acinetobacter baumannii , Providancia  rettgeri , Burkholderia cepacia  , Klebsiella pneumonia , Aeromonas salmonicida , Ochrobactrum anthropie .

Levofloxacin antibiotic was used in preparation of the hybrid nano compound after intercalating on layers of double hydroxide  (Mg/Al-LDH) . FT-IR spectrum studies has shown the appearance of specific chemical groups and the disappearance of others while the spectrum of x-ray (XRD) has shown the appearance of new levels of diffraction in the spectrum of the hybrid nano antibiotic Mg/Al-LEV-LDH as compared to the spectrum of the Mg/Al-LDH, which indicate that the prepared compound under study is a nano hybrid compound . Results of the Atomic Force Microscope (AFM), has shown that the diameter of the particles of hybrid nano antibiotic was 94.83 nm.

The inhibitory activity of the hybrid nano antibiotic Mg/Al-LEV-LDH and the free antibiotic (Free LEV) has been studied and the results revealed followings:

The hybrid nano antibiotic has the highest inhibitory activity against the bacterial isolate P. mirabilis 13b with an inhibition diameter of  34.75 mm , while the inhibition diameter for the free antibiotic was 47 mm against the isolate  17a .

The hybrid nano antibiotic showed the highest inhibitory activity against the isolate E. coli 14b with an inhibition diameter of 13.75 mm , while the highest inhibition activity for the free antibiotic was against the same isolate with an inhibition diameter of 26.5 mm .

The hybrid nano antibiotic showed the highest inhibitory  activity against the isolate P. aeruginosa 73 with an inhibition diameter of  41.5 mm , while the highest inhibition activity for the free antibiotic was against the same isolate with an inhibition diameter of 47.5 mm .

The highest inhibition activity for the hybrid nano levofloxacin was against the two isolates  S. aureus 2 and 3 with an inhibition diameter of 36 mm, for each, while the highest inhibition activity for the free levofloxacin was against the isolate S. aureus 3 with an inhibition diameter of 43 mm .

The combination  between the hybrid nano antibiotic LEV-LDH prepared in this study and free antibiotic Cefixime exhibited synergistic effect  against some P. mirabilis isolates while antagonistic effect has shown against the other isolates .

The combination  between the hybrid nano antibiotic LEV-LDH prepared in this study and free antibiotic Teicoplanin exhibited synergistic effect against some S. aureus isolates while antagonistic effect has shown against the other isolates .