دراسة تأثير الكيتوسان النانوي المرتبط ببعض العقاقير على خلايا سرطان الرئة

Rp. PStudying The Effect of Nano Scale Chitosan Bound to Some Drugs on Lung Cancer Cells pdf.

Abstract
This study aimed to prepare and know the effect of chitosan nanoparticles loaded with drugs (Mefenamic acid, Cephalexin and Paracetamol) on some chemical properties and study their effect in inhibiting the growth of lung cancer cells.

Its chemical properties were studied, including the solubility of nano-chitosan loaded with some medicinal drugs in different solvents (H2O, acetone, ethanol, hexane, DMSO, chloroform and coloring).
The absorbance (Abs.) and drug release were measured using a UV-Vis spectrophotometer in four different Buffer solutions with pH= (2.2, 6.0, 7.2, and 8.0 ) and at a constant temperature of 37oC as a function of time (hour and day).
The binding of nano-chitosan drugs to the amino acids present in the proteins of the lung cancer cell line type (A549) was studied, and the binding sites were predicted, and the most effective binding energy of nano-chitosan drugs was studied. Knowing the types of bonds between chitosan nanodrugs and amino acids.
The effect of drugs loaded on chitosan nanodrugs (B1, B2, and B3) was measured in inhibiting the spread of lung cancer, and the gene expression of the p53 gene was measured before and after exposure of lung cancer cell line type (A549) to chitosan nanodrugs. The results showed that the drugs have an effective effect In inhibiting the spread of cancer cells. The results of the current study showed a low toxicity value in the drug (B2) compared to other drugs. The (IC50) of nano-loaded chitosan drugs in preventing the spread of lung cancer was in the following order:
عقار الكيتوسان النانوي B2 > B 1> B3 >
زيادة كفاءة التثبيط عند IC50
The results also showed an increase in the level of gene expression when the lung cancer cell line type (A549) was treated with drugs, where the highest level was in drug (B2). It was observed that the p53 gene started functioning  normally and began to stop abnormal cell divisions