دراسة تأثير المستخلص المائي لأوراق نبات الهندباء في بعض المعايير الفسلجية والنسجية Taraxacum officinale في ذكور الأرانب المعاملة بمادة كلوتاميت احادي الصوديوم

Study The Effect Of Aqueous Extract Of Taraxacum Officinale On Some Physiological And Histological Parameters In Male Rabbits Treated With Monosodium Glutamate

Summary

The current study aimed to know the effect of the protective role of the aqueous extract of Taraxacum officinale (dandelion) leaves to reduce the toxic effect of monosodium glutamate (MSG) in white male rabbits, Lepus arcticus, and to evaluate its effect by studying some physiological, biochemical and histological parameters. The study was conducted in the College of Education for Pure Sciences/Department of biology Sciences and at home for the period from the beginning of October 2020 to March 2021. The study included (36) adult white male rabbits, their ages ranged from (6-12) months and their average weights ranged between (2000-1,500)grams. The rabbits were randomly divided into six groups (six rabbits per group) and dosed orally every day for a period of one month as follows: the first group (G1) dosed 2 ml of normal saline, the negative control group and the second group (G2) dosed at a concentration of 15 mg/kg of monosodium glutamate which was considered a positive control group , the third group (G3) was positively controlled and dosed at a concentration of 250 mg/kg of aqueous extract of dandelion leaves, the fourth group (4G) Dosed 250 mg/kg of dandelion aqueous extract, and four hours later dosed with MSG at a concentration of 15 mg/kg, the fifth group (5G) dosed with a concentration of 500 mg/kg of dandelion aqueous extract, and the sixth group (6G) dosed with a concentration of 500 mg/kg of extract. dandelion water, and after four hours, they were dosed with MSG at a concentration of 15 mg / kg of body weight. The animals were sacrificed after the end of the experiment period. Blood samples were collected after the end of the dosing period, and blood serum was obtained for the purpose of measuring the level of the following physiological, biochemical and histological parameters: – Hemoglobin (HB),White Blood cell (WBC),Red Blood cell ( RBC), as well as liver enzymes: alanine transaminase(ALT), aspartate transaminase(AST), alkaline phosphatase (ALP), blood sugar. total cholesterol (TC) cholesterol, triglycerides (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) and total protein (TP) and albumin measurements, globulin and Glutathione(GSH), Malondialdehyde (MDA). The study included measurement of histological changes in the liver, These include measuring the average diameters of hepatocytes, central veins, and sinusoids. And I took tissue sections of the liver. Oral administration of MSG-treated rabbits and aqueous extract of dandelion leaves physiologically led to: There was a significant increase (P≤0.05) in the mean level of ALP, AST, ALT, MDA, WBC, LDL, TG, TC, blood sugar in the MSG-positive control group in comparison with the negative control group. a significant decrease (P≤0.05) ) in the mean level of total protein albumin, globulin, GSH, HDL, HB, RBC in the MSG-positive control group compared to the negative control group. There was a significant increase (P≤0.05) in the average level of GSH, RBC and a significant decrease (P≤0.05) in the average level of blood sugar, ALP Globulin, WBC, LDL, TG, in the aqueous extract of dandelion leaf group at a concentration of 250 mg/kg compared to the positive control group. There was a significant increase (P≤0.05) in the average level of GSH, HDL and a significant decrease (P≤0.05) in the average level of TP, LDL, TG, TC, blood sugar, and ALP. Globulin, albumin in the group of aqueous extract of dandelion leaves at a concentration of 250 mg/kg and treated with MSG in comparison with the positive control group. There was a significant increase (P≤0.05) in the mean levels of HDL, RBC, HB, and GSH and a significant decrease (P≤0.05). In the mean level of blood sugar, TC, TG, LDL, ALP, AST, ALT, WBC, MDA, in the group of aqueous extract of dandelion leaves at a concentration of 500 mg / kg compared to the control group. There was a significant increase (P≤0.05) in the mean level of HDL, RBC, GSH and a significant decrease (P≤0.05) in the mean level of blood sugar, TC, TG, ALT, AST, ALP, LDL, TP, albumin, globulin, and MDA in the group of aqueous extract of dandelion leaves at a concentration of 500 mg / kg and substance treatment compared to the control group. Oral administration of MSG-treated rabbits and histologically aqueous extract of dandelion leaves led to: There was a significant increase (P≤0.05) in the mean diameters of hepatocytes, central veins and sinusoids in the positive control group compared with the negative control group. No significant differences (P≥0.05) in the mean diameters of hepatocytes and central veins Sinusoids and hepatic sinusoids in dandelion leaf aqueous extract group (250-500) mg/kg, as well as no significant differences (P≥0.05) in average diameters of hepatocyte and central veins, Sinusoids and aqueous. extract group with concentration (250-500) mg/ kg treated with MSG in comparison with the control group. The results of the histological examination of the liver led to histological changes represented by the presence of congestion and expansion of the central veins and a change in the shape of the liver cells and sinusoids in the positive control group compared to the negative control group whose sections are characterized by normal liver tissue with the integrity of the liver in the remaining groups. We conclude from the current study that the aqueous extract of dandelion leaves has significant effectiveness in reducing the toxic effects resulting from dosing male rabbits with MSG on the biochemical, physiological parameters and liver tissue due to the presence of antioxidants available in dandelion leaves, which gives protection to the blood. We conclude from the current study that the concentration of dandelion plant 500 mg / kg, which gives better physiological and histological results than the concentration of 250 mg / kg.