دراسة جينات المقاومة للمضادات الحيوية والمعزولة من بعض الأجناس البكتيرية المرضية وتحديد تأثير البكتيريا المعززة حيويا والمشخصة جزئياً

Molecular Study Of Antibiotics Resistance Genes of Some Pathogenic Bacterial Isolates And Determination The Effect Of Probiotic Bacteria Which Molecular Identified

The summery
Probiotics are living microorganisms that provide many benefits to humans, including Lactobacillus that are naturally present in the human body and in some types of foods, drinks, and nutritional supplements.
In this study, the antagonistic activity of some types of Lactobacillus against some types of bacterial pathogens isolated from different clinical sources was determined. One hundred and ten samples were collected from burn infections, urinary tract infections, wound infections, and discharge from both sexes at Imam Hussein (peace be upon him) Teaching Hospital, from the burns lobby and from the Obstetrics and Gynecology Hospital, and cultured on appropriate media. Phenotypic and biochemical tests were then conducted to diagnose pathogenic and bacteria. Polymerase chain reaction method was applied to detect genes encoded for antibiotics resistance activity that including (shv, bla Tem, ermA, , aac (6′)-Ib – cr qnrA). On the one hand, 20 different samples of raw milk and homemade yogurt were collected to isolate Lactobacillus spp., which were diagnosed by microscopic, biochemical and PCR method. Finally, the antagonism of Lactobacillus against pathogenic bacteria was then tested and included:
1.Lactobacillus. acidophilus+ Lactobacillus. rhamnosus
2.Lactobacillus. acidophilus + Lactobacillus. hellveticus, 3.Lactobacillus.acidophilus + Lactobacillus. plantarium, 4.Lactobacillus.rhamnosus + Lactobacillus.hellveticus,
5.Lactobacillus. rhamnosus + Lactobacillus.plantarium,
6.Lactobacillus. rhamnosus+ Lactobacillus. Acidophilus
7. Lactobacillus. hellveticus+ Lactobacillus. plantarium
Later, the cell filtrate of LAB was used to evaluate the biological effectiveness of LAB against pathogenic bacteria. Finally, the synergistic interaction between each species of Lactobacillus and polysaccharides of the type Fructooligosaccharides against each genera of bacterial pathogens was individually investigated. In all aforementioned trails, the method of diffusion from wells was used in all experiences..
In the results: 5 isolates of Staphylococcus aureus, 7 of E. coli, 3 of Pseudomonas aeruginosa, and 9 of Klebsiella pneumonia were obtained. The mentioned bacterial species were resistant to a wide range of antibiotics as Klebsiella bacteria were characterized as having the highest significance in their possession of the aac(6′)-Ib -cr gene, which encodes resistance to a group of aminoglycoside antibiotics (P<0.05) compared to the rest of the bacterial genera, while Pseudomonas aeruginosa bacteria were significantly less in possession of two isolates of this gene. . As for the erma gene, the results were similar to the aac(6′)-Ib -cr gene. Klebsiella bacteria carried the qnrAA gene, which encodes resistance to a group of fluoroquinolone antibiotics, with 4 isolates significantly higher than the rest of the bacterial genera, as these genera were equal to each other, with one isolate from each species carrying this gene.
Also, Klebsiella bacteria were the only ones carrying the shv gene, which encodes resistance to a group of beta-lactam antibiotics, while the rest of the genera did not possess this gene. As for the Bla Tem gene, which encodes resistance to a group of beta-lactam antibiotics, the results did not differ significantly from the results of the shv gene, except for the presence of one isolate of the Pseudomonas aeruginosa bacteria carrying this gene, while Staphylococcus aureus and Escherichia coli bacteria did not carry this type of gene.
In addition, 4 species of Lactobacillus were isolated including L. rhamnosus, L. hellveticus, and L. acidophilus. L. plantarium,
All types of LAB alone either overnight culture or supernatant overnight culture were not exhibited any antagonistic activity against bacterial pathogens. Moreover, the mixed of two species of LAB was also not revealed any action against tested pathogens. Testing of the antagonistic activity of LAB with FOS showed that the growth of pathogenic bacteria was affected by this mixture. Conclusion: There was a significant inhibitory ability for all types of LAB after mixing them with FOS, which led to improved growth of Lactobacilli.