دراسة مقارنة لتأثير المستخلص المائي لمادتي Commiphora molmol L. وBoswellia serrata L. على بعض المعايير الكيموحيوية والنسجية في ذكور الجرذان النرويجية البيض المستحث بها الفشل الكلوي

A comparative study of the effect of aqueous extract of Commiphora molmol L. and Boswellia serrata L. On some biochemical and histological parameters in male Norwegian white rats induced renal failure

This study aimed to find out the effect of the aqueous extract of Commiphora molmol L. and Boswellia serrata L. on some of the biochemical and histological parameters of the kidney in male Norwegian white rats induced renal failure.
This study was conducted in the animal house of the Department of Life Sciences / College of Education for Pure Sciences / University of Karbala, and was carried out during the period from November 2017 to October 2018, male laboratory rat of the Norwegian type Rattus norvegicus was used in this study. The animal of the Department of Life Sciences / College of Science / University of Dhi Qar, and their ages ranged between (8-10) weeks and their average weight ranged between (200-240) grams. These animals were divided into 8 groups, 7 groups were treated with CdCl2, with a concentration of 0.6 mg / kg of body weight per day for a period of six weeks to induce the outbreak Chronic Renal Failure (CRF) and one group remained without treatment as a negative control group.
In the experiment, 120 adult male rats were divided randomly into 8 groups of equal numbers, each group included 15 rats, and by 5 animals per group, each group was treated with one of the plant concentrations, knowing that one group was divided into three sub groups according to the duration of the dose (a week, Two weeks, three weeks) After the induction of the disease, the animals were fed with aqueous extracts of the Myrrh gum plants, the Kundur gum, and in concentrations (10, 20 and 30) mg / kg of body weight in addition to the negative, positive control group.
The results of the detection of the effective compounds in the aqueous extract of the myrrh and Kundur gummies indicated the presence of a number of active compounds using various chemical reagents, as specific disclosures revealed that the extracts contain a number of effective compounds such as: tannins, saponins, cyclosides, turbines, steroids and flavonoids, and a technique has shown (HPLC) High Performance Liquid Chromatography for the Detection of Effective Phenolic, Flavonoids and Resins of the Aqueous and Bacterial Colloidal Aqueous Extract.
Blood samples and nephrectomy were collected from control groups and treated with cadmium chloride and groups treated with the aqueous extract of myrrh and kundur gum after induction of chronic renal failure, to study some biochemical parameters that included measuring the level of each of: Kidney Injury Molecule-1 (KIM-1), Cystatin C (Cys-C), Glomerular Filtration Rate (GFR), Neutrophil Gelatinase-associated Lipocalin (NAGL), creatinine, urea, blood urea nitrogen (BUN), albumin protein, study of pathological histopathological changes in the kidney with the optical microscope and microscope Electronic transmitters of influencing potion groups.
The results of the current study showed that the induction of chronic renal failure with cadmium chloride led functionally and histologically to a significant decrease (P <0.05) in the level of GFR, and a significant increase (P <0.05) in creatinine level, Urea, Blood urea nitrogen, and KIM-1 Albumin, Cys-C, and NGAL for all concentrations and periods compared to the control and treatment groups. The results also indicated that grouping the groups treated with the aqueous extract of the Myrrh and Kundur membranes resulted in a significant increase (P <0.05) in the GFR level, and a significant decrease (P> 0.05) in Creatinine, Urea, Blood Urea Nitrogen Level, Albumin, KIM-1, Cys-C, Comparative NGAL E with the positive and negative control groups.
The induction of chronic renal failure resulted in histological changes in the kidneys of the treated animals compared to the control group as changes in the kidney tissue occurred with blood congestion, glomerulonephritis, Necrosis, renal tubular degeneration, infiltration of inflammatory cells, and the presence of protein substances in Renal tubules, Bowman’s Expansion of Bowman’s Capsule, Increased Cellular Glomerulus, while electron microscopy results showed that the accumulation of cadmium chloride was Damaged by the renal tubules, regression decline, cytoplasm degeneration, destruction of the destroyed nucleus, agglomeration of chromatin, edema For mitochondria, the difference in their sizes and shapes, some of which are destructive, Destroyed, and regression of the lining of norms Cristae Endothelium in it.
It can be concluded from the above results that treatment with the aqueous extract of Myrrh and Kundur is a positive effect in repairing the damage caused by Cadmium poisoning to many biological and estrogen-related organisms and organelles Adverse effects and the presence of a clear improvement in the kidney tissue by reducing degenerative and glomerular changes of a radius close to normal, and an improvement in cell organelles as well as an increase in their ability to act as antioxidant and stimulating immunity factors, as well as anti-inflammatory.