دور كيموكاين CXCL10 وعوامل ضراوة لبكتريا E.coli المعزولة من مرضى التهاب المجاري البولية

Role of Chemokine CXCL10 and Virulence Factors of E.coli isolates From Patients with Urinary Tract Infection

Abstract

Urinary tract infections (UTI) are one of the most prevalent bacterial infections affecting millions of individuals throughout the world each year.
The two types of urinary tract infections are categorised based on where they occur. The upper urinary tract is impacted by pyelonephritis, which affects the ureter and parenchymal kidney, and the lower urinary system is affected by cystitis and urethritis, which affects the bladder or urethra.
A case control study was conducted for period of 5 months, starting from September(2023) to January (2024) at Al-Hussein Teaching Hospital / Laboratory Microbiology in Karbala, Iraq; the total number of participants were 145 Subjects; they were divided into two groups: the first one includes 100 patients with UTIs, the second group includes (45) as healthy control.
Urine samples were taken from UTIs patients and performed for Aerobic bacteriological examination profile (microscopically ,culturing morphologically, and confirmative test by VITEK 2 compact system).C-X-C Chemokine ligand 10 levels detected in urine by serological techniques of sandwich ELISA.

Bacterial species isolated from UTIs group represented by the Gram positive bacteria were Coagulase –v e staphylococcus 9 (20%), the commonest isolated species followed by Staphylococcus haemolyticus4(8.9%), Staphylococcus aureus 4 (8.9%), Staphylococcus epidermidis 2(4.4), Staphylococcus hominis 1(2.2%), Enterococcus faecalis 1 (2.2%), and
Gram negative bacteria that represented by Escherichia coli (35.6%), were the most common followed by Klebsiela pneumonia 3(6.7%) and Enterobacter aerogenes 3(6.7%).
All study isolated of E.coli uropathogens were confirmed by molecular detection using specific 16srRNA.
The percentage of virulence factors genes distribution in 16 isolates E.coli represented FimH gene 16 (100%), OmpT gene 8(50%), Pap gene 9(56.3%), KpsmII gene 13 (81.3%).
According to antibiotic sensivity which performed by vitek2 compact system some isolate E.coli showed a multi-drug resistance patterns (Multidrug- resistant, Extensively drug-resistant) level of resistance to the tested antibiotics, with a rate of 50%, where MDR(Multidrug- resistant) formed (25%),and XDR (Extensively drug-resistant) formed(25%).The common antibiotics sensitive for UTI treatment Meropenem, Imipenem, Gentamicin are used for treatment of UTI infected by E.coli.
Current study revealed that (C-X-C motif chemokine ligand 10 )level was highly significant difference (P < 0.001) in all patients group incompared with healthy control.
This study showed that E. coli is the most predominant uropathogen of bacteria UTI(35.6%) followed by Coagulase –ve staphylococcus(20%). On the other hand, Urine chemokine 10 (CXCl10) have important roles in the diagnosis of UTI infection. E.coli uropathogen isolates showed many virulence factors and also showed Middle rates of resistance to different antibiotics (MDR,XDR).