الدور العلاجي لمستخلص الكحولي لنبات الحلبة ضد السمية الكبدية الكلوية المستحدثة بماده الاسيتامينوفين في ذكور الجرذان (Trigonella Foenum graecum)

Therapeutic Role of Fenugreek (Trigonella Foenum Graecum) plant Alcoholic Extract Against Hepatonephrotoxicity Induced by Acetaminophen

Abstract
The present study was conducted at College of Veterinary Medicine /University of Kerbala. The study is performed during the period from December, 2021 to March, 2022. The current study was proceed to aimed to detect the therapeutic Role of Trigonella Foenum Graecum(TFG). Forty male rats were divided equally into 4 groups as the following: 10 healthy rats as control negative (C-) group given distilled water along of experiment, C+, T1 and T2 groups experimentally induced nephrotoxicity by acetaminophen oral administration for 14 consecutive days. After that T1 and T2 received 150BW and 300BW of Fenugreek leave extrae respectively for 14 consecutive days while control positive group (C+) leave without any treatment .
The results showed significant depletion in serum creatinine in groups treated with FSE extract in dose dependent manner. In contrast the high levels of Blood Urea Nitrogen (BUN) and creatinine revealed in control positive group which received 2BW of acetaminophen without treatments. For the iron levels there were also significantly increased in animals exposed to FSE in both doses 150 and 300BW comparing with control negative, furthermore there was increase in group of control positive which was healthy animals comparing with those exposed to ace taminophen toxicity.
Significant increase (P ≤ 0.05) in blood parameters such as Red Blood Cells (RBC), MCH% , , Hb, Granulocyte and MID%, in animals groups were treated with FSE. On the other hand WBCs and lymphocytes significantly (P ≤ 0.05) decrease in T1 and T2 groups when compared with C+ group.
Histopathological changes showed significant damage and loss of architecture on renal tissue and remarkable glomerular necrosis in C+ animals’ group. On the contrary, the supportive effects of fenugreek extracts 300BW clearly appeared in kidney and liver section showed normal tissue.
In conclusion, fenugreek extract has good therapeutic effects on biochemical and hematological parameters depending on dose and concentration of extract as well as arise clearly in nourishment of kidney and liver tissue.