الدور الوقائي لمسحوق الطماطم على بعض المعايير الفسلجية في الجرذان المستحدث بها الفشل الكلوي المزمن

The protective role of Tomato powder on some physiological parameters in Rats induced Chronic Renal Failure

The present study was conducted at College of Veterinary Medicine /University of Kerbala. The study is performed during the period from November, 2017 to April, 2018, to evaluate some coagulation factors, biochemical parameters, liver and kidney histological changes in rat which induced- chronic renal failure and possible protective role of tomato powder against chronic renal failure. Twenty four male rats (Rattus norvegicus) where used in the study, divided equally and randomly in to four groups, First group control animal has been fed with normal diet and injected intraperitoneally with Dimethyl sulfoxide (DMSO). Second group rats had been injected with adenine 50 mg/ kg B.W. suspended with Dimethyl sulfoxide (DMSO) , for 4 weeks induced chronic renal failure and fed with normal diet, the third and fourth group rats had same protocol used in second group except they were feed with 10% and 20% Tomato Powder respectively.
The study was aimed to determine the effect of renal failure on blood coagulation factor (fibrinogen, factor VII and factor VIII), estimation of kidney function (Kidney injury molecule -1, urea and creatinine). Estimation of Serum Alanine Aminotransferase Activity(ALT) and Serum Aspartate aminotransferase Activity(AST), estimation of antioxidant enzyme like Glutathione-s- transferase (GST) and Superoxide dismutase (SOD), estimation the of blood picture parameters and histological section of kidney and liver was also performed.
The results showed a significant increase (P ≤ 0.05) in coagulation factors like: fibrinogen (common pathway of coagulation), Factor VII (extrinsic pathway) and Factor VIII (intrinsic pathway), and increase in kidney and liver function parameters as kim-1, urea creatinine, Serum Alanine Aminotransferase Activity(ALT) and Serum Aspartate aminotransferase Activity(AST) in rats treated with adenine-induced renal failure compared with the control group.
Significant decrease (P ≤ 0.05) in blood parameters such as RBC, PCV, Hb, Platelet, Granulocyte and Lymphocyte, and significant decrease (P ≤ 0.05) in some antioxidant enzymes Glutathione-Stransferase (GST) and Superoxide dismutase (SOD), but elevation of WBC in rats induced chronic renal failure in comparison with control group. Histology of liver and kidneys in group of rats treated with adenine, showed a significant damage necrosis such as inflammation and fibrosis. On the other hand, the liver showed infiltration of hepatocyte with inflammatory cell, and enlargement in the portal vein in adenine group compared with control group.
The results showed a significant decrease (P ≤ 0.05) in coagulation factors like: fibrinogen (common pathway of coagulation), Factor VII (extrinsic pathway) and Factor VIII (intrinsic pathway) in rats treated with adenine and Tomato powder 10% and 20%, and decrement in liver and kidney function parameters such as kim-1, urea creatinine, AST and ALT compared with the adenine group. Significant increase (P ≤ 0.0.5) in blood parameter such as RBC, PCV, Hb, Platelet, Granulocyte and Lymphocyte, and significant increase (P ≤ 0.05) in some antioxidant enzymes (SOD and GST) are noticed, but decrease of WBC in groups induced chronic renal failure and feed with Tomato powder at dose10% and 20% is seen compared with adenine group.
This study conclusion that, feeding tomato powder at 10% and 20% dose contributed to improve some parameters associated with chronic renal failure mainly due it’s effect as antioxidant, and these results are shown clearly. However, The effect of tomato powder at 20% showed improvement higher than 10%diet.