تأثير الزايليتول في بعض الحركيات الخلوية قبل الالتهابية المستحثة بعديد السكريد الشحمي من بكتريا Aggregatibacter actinomycetecomitans

Xylitol effect in some pro-inflammatory cytokine induced by Lipopolysaccharide from bacteria Aggregatibacter actinomycetecomitans

ABSTRACT
Collected 70 samples from patients with infections Periodontitis and the auditors of the Center for Health Specialist Dental in Karbala and for the period between February and June 2013, Bacteria diagnosed by testing Morphological and Biochemical , Tthe rate of infection Aggregatibacter actinomycetecomitans is ( 28.5 % ) . The present study aimed to know the effect of Xyilitol in inhibiting the growth of bacteria and induced Lipopolysaccharide (LPS) extracted from the bacteria using Extraction Kit in some of pro-inflammatory Cytokine each of the Interleukin IL-1β , and Tumor necrosis factor alpha TNF-α. Through In vivo testing by local injection LPS in two doses in the male rats gingiva which were used for the experimental study and that divide them into three groups injected first with LPS, and injected Other Xylitol with LPS, either recent was injected with Normal saline as Control by maturing levels of technology Enzyme Linked Immunosorbent Assay (ELISA), The results showed significant differences between the groups, and increased levels of cytokine in the serum of rats after 24 and 48 hours of injection , where it was observed that the highest value recorded in the group treated with LPS was (333.50 & 314.83 ) Pico g / ml for cytokine IL- 1β after 24-48 hours , respectively, compared with Control ( 188.33 & 181.00 ) Pico g / ml for the first dose was either the second dose ( 317.83 & 338.83) Pico g / ml ,the study showed also Xylitol may inhibits LPS for IL-1β in the group treated Xylitol with LPS and get significant decrease when compared to the group LPS and Control , which were (266.66 & 262.66 ) Pico g / ml for the first dose , while the second dose was (253.38& 258.94 ) Pico g / ml , and for TNF-α results showed significant differences in the levels in the group treated with LPS of two doses the first was ( 151.66 & 154.00 ) Pico g / ml after 24 – 48 h , respectively , compared to the Control , which was ( 120.00 & 124.66) Pico g / ml , and the second was hit (167.66&162.33 ) Pico g / ml , compared with the Control was ( 122.33 & 124.66 ) Pico g / ml , Xyitol showed inhibiting action in LPS induced TNF-α in group treated Xylitol with LPS and get significant decrease for first dose was (137.33&140.66) Pico g / ml , while second dose was ( 142.00 & 143.66 ) Pico g /ml .
Study showed that Xylitol works to inhibits growth of bacteria A. actinomycetecomitans ,20% concentration was focus most of effectiveness, thus conclude that the effectiveness of xylitol inhibition against LPS induced production of pro-inflammatory cytokine and growth of bacteria A. actinomycetecomitans …..