تأثير تجميد النطف السريع والإذابة وأضافه مضادات الأكسدة في مستويات الجذور الحرة ومعايير النطف لمرضى قليلي الخصوبة

Effect of Rapid Freezing and Thawing and Antioxidants addition on Free Radicals levels and Sperm Parameters of sub- fertile Patients

This study was performed in the laboratory of Intra Cytoplasmic Sperm Injection and freezing of sperm in the fertility center of Al-Sader Medical City /    Al-najaf Alashraf City during the period from May, 2012 to April 2013.     The study aimed to study the effect of rapid freezing and thawing processes on semen and sperm parameters, Malondialdehyde concentration ( MDA) and abnormal sperm chromatin percent, besides to study the effect of adding types from antioxidants Including vitamin E in concentrate 40  and Glutathione in concentrate 1 mm to the sperm freezing media (Sperm Freeze) In minimizing the negative effects which result from sperm rapid freezing and thawing processes.

     The study included three types of Infertility patients Normozoospermic, Oligozoospermic, and Asthenozoospermic patients. The Number of patient’s was (30) in each case .  The Mean of age of Normozoospermic patients were (32.2+_1.31) year, Oligozoospermic patients (31.1+_1.29) year and, Asthenozoospermic patients (33.1+_1.23) year. The results showed that the sperm activation processes  lead to a significant difference (p<0.05) in sperm parameters in comparison with these before activation . The present study results showed that sperm activation by using simple layer technique  to patients samples of  Normozoospermic  and Oligozoospermic patients lead to a significant decrease (p<0.05) in sperm concentration, round  cell concentration , Malondialdehyde concentration and abnormal sperm chromatin percent Means  and  a significant increase (p<0.05) in sperm motility forward movement percent , Sperm viability percent and normal sperm morphology percent in comparison with their values before activation  . While the sperm activation processes of Asthenozoospermic patients by useing Centrifugation Wash – Out technique lead to a significant decrease (p<0.05) in sperm concentration, round cell concentration and abnormal sperm chromatin percent Means and a significant increase (p<0.05) in sperm motility forward movement percent, sperm viability percent, normal sperm morphology percent and Malondialdehyde concentration In comparison with their values before activation.   .                                                                                                      The present study results showed that sperm rapid  freezing processes of patients who were included  in the study  by using sperm freezing media ( Sperm Freeze) and  thawing processes after one month from freezing leads to a significant decrease (p<0.05) In the sperm concentration, sperm motility forward movement percent, sperm viability percent and normal sperm morphology percent. and a  significant increase (p<0.05) in the Malondialdehyde concentration and  abnormal sperm chromatin percent and no significant difference(p<0.05)  in round  cell concentration in comparison with their values  after activation .

     Study of  freezing time by calculating sperm  parameters in the first month and compared it with second and third months  after freezing and thawing in the samples of patients who were included  in the study showed  there was a there was a significant decrease (p<0.05) in the sperm motility forward movement percent and sperm viability percent  ,and there was a significant increase (p<0.05) in the Malondialdehyde concentration and  abnormal sperm chromatin percent and no significant difference(p<0.05) in the sperm concentration, normal sperm morphology percent and round cell concentration Means in the second and third month  In comparison with their values after doing the thawing processes after one month from freezing .   The study results shows a significant decrease (p<0.05) in the sperm motility forward movement percent and sperm viability percent  ,and there was a significant increase (p<0.05) in the Malondialdehyde concentration and abnormal sperm chromatin percent and no significant difference(p<0.05) in the sperm concentration, normal sperm morphology percent and round cell concentration when comparing third month Means with  second month Means.                                        .                                                                                                              The study results shows that used of sperm Freezing Media ( Sperm Freeze) with adding antioxidants like  vitamin E or Glutathione in single form or in the mixed form from the tow antioxidants leads to a significant difference (p<0.05) in sperm parameters of study included patients , there was a significant increase (p<0.05) in the sperm motility forward movement percent s and sperm viability percent  ,and  there was a significant decrease (p<0.05) In The Malondialdehyde concentration and  abnormal sperm chromatin percent and no significant difference (p<0.05) In the sperm concentration, normal sperm morphology percent and round cell concentration In comparison with their Means when used of sperm freezing media ( Sperm Freeze) alone .                .                                                            It can be concluded  from this study  that  sperm  rapid  freezing and thawing processes had a negative  effect on the semen and sperm parameters and Malondialdehyde concentration and abnormal sperm chromatin percent in all samples  of study included patients  , and this negative  effect of rapid  freezing and thawing processes increased with increasing of freezing time , and Adding  of antioxidants vitamin E or Glutathione in single form or In the mixed form shows resistance to this negative  effect  leads to a significant increase (p<0.05) In the studying  parameters  .