تأثير مسحوق اوراق المورينغا على مؤشرات ايض الحديد في ذكور الجرذان المصابة بالفشل الكلوي المزمن المستحدث

The effect of Moringa leaves powder on iron metabolism indices in male rat with Induced chronic Renal failure

Our study was performed at faculty of Veterinary Medicine/University of Kerbala. It is performed during the period from1 november 2019 to 1 February 2020 ) .The present study was designed to investigat, the relation between Erythroferrone (ERFE) in iron homeostasis and erythropoietic activity in anemia related with induced chronic renal failure in male rats via investigation the correlation between the ERFE and other hormones such as erythropiotein ,hepcidin ,ferritin ,estimation the Hb,PCV,RBC , serum iron,determination the of kidney functions test (creatinine ,urea), and histopathological changes in kidney ,liver and spleen.
Sixty male rats were randomly divided into (4/groups) for 42 days , the first group (GI) control negative was with dimethyl sulfoxide by interaperitoneally for 4 weeks ,The second group (GII) is the positive control was administrated with DMSO by interaperitoneally for 4 weeks and then moringa leaf powder given at dose rang 5% for 2 weeks with diet, the third group (GIII) was administrated interaperitoneally 100mg/kg.bw for 4 weeks for induction of renal failure and fourth group (GIV) adenine were administrated interaperitoneally 100mg/kg.bw for 4 weeks for induction of renal failure and the given moringa oleifera leaf powder at dose 5% for 2 weeks with diet .
The results showed there was a significant elevation in the serum urea , serum creatinine,serum ferritin and serum hepcidin in (GIII) adenine treated group and statistically significant decrease in RBC count ,PVC and HB in adenine group (GIII) in addition to decrease serum erythropoietin, serum erythroferrone and serum iron in comparsion with the other groups ,after moringa oliferia leaves administration we observe that there was a significant reduction in serum urea ,serum creatinine ,serum ferritin and serum hepcidin in (GIV) treated group and statistically significant (p≤0.05) increase in RBC count ,PVC and HB in Forth group (GIV) in addition to serum erythropoietin, serum erythroferrone and serum iron in comparing to (GIII) group .
Histological changes in kidney , spleen and liver demonstrate that adenine treated group was damaged atrophied and degeneration especially the renal tubules that have the adenine crystalline precipitation in add to inflammatory infiltrate ofinflammatory in comparing to control group howere after moringa olifera leaves administration the tissues are able minimize the inflammatory condtion but not fully recoverd .
This study explains the induction of renal failure by adenine and how can chronic renal failure results in anemia and the ablitity of erythrobiotein hormone effect in a positive and negative correlation with the studied biomarkers also explains how can moringa olifera leaves powder works on improvement of anemia