تقييم الانترلوكينات 6 و 10 و EG-VEGF في وقت سحب البيوض وارتباطها بنتائج دورة التلقيح الصناعي والحقن المجهري

Evaluation of IL-6, IL-10 and EG-VEGF at time of oocytes retrieval in correlation with the outcome of IVF-ICSI cycle

Abstract

Infertility is defined as the inability to achieve pregnancy after 12 month or more of unprotected sexual intercourse. Ovulation disorders, such as Polycystic Ovary Syndrome (PCOS) and Poor Ovarian Reserve (POR), are among the female factors contributing to infertility. The ovarian tissue, along with immune cells, produces cytokines, which play a crucial role in follicular growth, steroidogenesis, leukocyte recruitment, and tissue remodeling during ovulation, luteinization, and luteolytic. This study aimed to evaluate the levels of Interleukin-6 (IL-6), Interleukin-10 (IL-10), and Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-VEGF) in follicular fluid and serum, and to investigate the correlation between these markers and the outcomes of Intracytoplasmic Sperm Injection (ICSI).
This cross-sectional study included subjects divided into four groups based on the cause of infertility: unexplained infertility, male factor, female factor, and combined factors. The study was conducted at Al-Kafeel Super specialty Hospital in Karbala from February 2024 to June 2024.
Serum and follicular fluid samples were collected on the day of oocyte retrieval, and the levels of IL-6, IL-10, and EG-VEGF were measured using the ELISA technique
The result of present study showed that FSH and LH were significantly higher in the female and combined factor groups compared to the male and unexplained factor groups, while E2 day2, AMH, and E2 on the day of injection HCG levels were lower in the female and combined factor groups compared to the male and unexplained factor groups. In addition, the total number of oocytes, fertilization rate, Embryo Grade I (GI), Embryo Grade II (GII), number of transferred embryos, and pregnancy rate in unexplained and male factor were high significantly different from the female and combined factor groups.

Regarding, the serum and follicular levels of cytokines, there were no significant differences were observed in the levels of IL-6, IL-10, and EGVEGF in follicular fluid and serum across the patient groups.
Furthermore, IL-6 levels in serum were significantly higher in pregnant women compared to non-pregnant women. In addition, EG-VEGF levels in follicular fluid showed a negative correlation with positive pregnancy outcomes.
Moreover, in this study there were positive correlations were observed between EG-VEGF in serum and Embryo Grade III (GIII), and between EG-VEGF in follicular fluid and Embryo Grade II (GII). In addition, Positive correlations were also found between IL-6 and EG-VEGF levels in serum and their corresponding levels in follicular fluid.