تقييم دورأوبيكينون (الإنزيم المساعد Q10 ) ضد التأثير السمي لل Busulfan في ذكور الجرذان

Assessment the role of Ubiquinone (coenzyme Q10) against to toxicity effect of Busulfan in male rats

Busulfan is a chemotherapy treatment for cancer, while coenzyme (ubiquinone CoQ10) is a coenzyme that works to enhance chemical reactions as antioxidant against free radicals in the body and It helps in decrease the effect of treating by chemotherapy drugs like Busulfan .
This study was conducted at Department of Physiology, Biochemistry and pharmacology in College of Veterinary Medicine / University of Karbala from December 2019 to end of February 2020. The role of ubiquinone was evaluated against the toxic effect of busulfan on sperm in adult albino mice at periods of 28 and 56 days.
Forty eight adult male rats were divided into four groups randomly and equally in each group of (12 rats for each group, animals feeding and the water was available for 8 weeks periods.
The first group was injected with distilled water as control group, second group was injected intraperitonially with single dose10 mg / kg of Busulfan (chemotherapy) ,while The third group injected intraperitonially by single dose of Busulfan 10 mg / kg and the coenzyme ubiquinone (COQ10) 10mg\kg daily ,and the fourth group injected intraperitonially with only CoQ10 10 mg / kg daily to the end of study.
The results showed a significant decrease (p ≤0.05) in hematological parameter (RBC,Hb,Pcv,Plt and WBCs)in both group G2 and G3 While No significant (p ≥0.05) inG4 as comparison to G1 in 28day study period. At 56 day ,the results showed a significant decrease (p ≤0.05) in hematological parameter with improvement in (RBC,Hb,Pcv,Plt and WBCs) in both group G2 and G3 While No significant (p ≥0.05) inG4 as comparison to G1.
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The Results of hormones indicated that testosterone ,ADPN,INH-B and ACV-A hormones has a significant decrease (p ≤0.05) in G2, but not significant (p ≥0.05) in G3 and G4 when compared with G1control group. While LH and FSH hormones were significant (p <0.05) increase in G2 and G3,but not significant (p ≥0.05) of G4 when compared with G1 Control group at two peroids study 28 and 56 days.
The Result of liver parameters showed that AST,ALT, ALP ,albumin and bilirubin has a significant increase (p ≤0.05) in G2 and G3, but not significant(p≥0.05) in G4 when compared with control group at two periods study 28 and 56 days. While results showed renal parameters Urea, Uric acid and creatinine has a significant increase (p ≤0.05) in G2 and G3, but not significant (p≥0.05) in G4 when compared with G1control group at two periods study 28 and 56 days.
The histological study showed a significant decrease in the diameter of the seminal tubes, as well as the occurrence of erosion of the testicle tissue in the animals injected with Busulfan (chemotherapy) compared to the control animals. Also observed changes in the kidneys, which is a decrease in the diameter of the glomeruli in animals treated with Busulfan compared to control animals. But when animals are injected with co 10 we notice an improvement in damaged tissues as a result of chemotherapy toxicity.