دراسة البروتينات المتفاعلة في المرحلة الحادة في مريض الإنتان

Study of Acute phase Reactant Proteins On Patients With Septicemia

Septicemia, or sepsis, is the clinical name for blood poisoning by bacteria. It is the body’s most extreme response to an infection. Sepsis that progresses to septic shock has a death rate as high as 50%, depending on the type of organism involved. Sepsis is a medical emergency that needs urgent medical treatment. Without treatment, sepsis can quickly lead to tissue damage, organ failure, and death. There are Several causes of infection including parasites, bacteria, fungi.
In this study One hundred (100) participants were enrolled sub divided in to three groups involved in this case-control study according to clinical diagnosis ,Patients were suspected to have septicemia by sign & symptom taken by physician which noted the information of patients file in the center ,]50 patients(8 neonatal , 42 adult)[ from both sex](14 female) ,(36 male)[ and divided two groups :first group A includes patients with positive blood culture] 23 (6 neonatal ,17 adult)[, the second group B includes patients with negative blood culture ] 27(2 neonatal ,25 adult)[ and the third group includes healthy control [50 (10 neonatal, 40 adult)] ,](11 female),(39 male)[ People were picked from the general public and appeared to be in good health,
(5-15 ml) of blood was aspirated from the veins of the patients. Blood sample of at least (9-10) milliliters (adults) and (5-8) milliliters (neonatal) was used for the blood culture ,taken (3 mL) of blood was deposited at room temperature in a gel tube for biochemical tests .The leftover blood sample (2ml) was placed in an EDTA tube and shaken for CBC , and ( 1.8 ml) blood sample was put in the sodium citrate tube for fibrinogen.
The purpose of the present study is to find the effect some biomarker on sepsis patients like acute phase reactant proteins and HS.troponin and Pro BNP, albumin,prealbumin,C-reactive protein,α1-antitrypsin, haptoglobin and fibrinogen, also identification of the common type of bacteria that occur in septic patient.This atudy conducted at Imam Hussein hospital in Center ICU & Imam Zain Al-Abdeen hospital during the period from October 2022 to May 2023.
The results found that total WBC of the patient groups ( A with bacterial growth B:without bacterial growth ) for neonate and adult significant higher than control group (C) at level P value (< 0.05) while there are no significant differences between adult and neonate in different groups A,B,C,of other parameters (lymphocyte, neutrophil, platelets) at level P value (< 0.05).
Biochemical parameter (CRP,serum amyloid A,Alpha 1antitrbsin,fibrinogen ,gamma globulin , Haptoglobin) of the patient groups ( A &B) for neonate and adult significant higher than control groups (C) at level P value (< 0.05). But in (Albumin ,ceruloplasmin ,Pro BNP ) of the patient groups ( A &B) for neonate and adult non-significant higher than control groups (C) at level P value (< 0.05), There is no significant differences between adult and neonate in different groups.
Found that type of bacteria of the sepsis patient groups for neonate and adult (21.75%) staphylococcus aureus, (17.40%) staphylococcus epidermidis (13.04%) Enterobacter aerogenes, significant higher than other type of bacteria staphylococcus warner (4.35%) respectively and there are no significant differences between adult and neonate in different groups.