دراسة التغيرات المناعية والوراثية في الأشخاص المعرضين للنفط ومشتقاته

Immunological and Genetic Changes Study at Persons exposed to petrolum and its derevatives

The Immunological and Genetic changes were studied  and  performed as a result of petroleum exposure and its derivates. for (54) workers who works in karbala they exposed to 3rd degree level of petrol compounds and its derivatives. their ages were ranging from (20-58)years compared with (20) randomly selected sample from healthy workers who were not exposed to petrol as standard sample at the period from

1/ 10 / 2004 to 1 / 12 / 2005.

Cytogenetic test to W.B.C for petroleum exposure workers by using the method of tissue Culture and Banding Technique compared with standard sample. The result of genetic test showed the occurrence numerical chromosomal changes were shown in two cases only,which their karyotype were (47, XY). the results referred also to a presence of numerical and structural complex chromosomal changes in one case, it’s karyotype  was : 47, XY, -17, -17, +der (17), -22, +der ( 22 ),+2dot mar

Mitotic index factor (MI) and Lymphoblast index factor (BI) were studied also, which they showed in general significant increase in petroleum exposure workers compared with standard sample, and when classified workers Were divided into two groups the first group smoker workers who spend more than 15 years in their jobs and their ages were more than 30 years old.

the second group non-smoker workers who spend less than 15 years in their jobs and their ages were less than 30 years old. i t was noticed that there was an increase in MI and BI in first group compared with the second group .

The results of present study showed that there were changes with a significant effect in decrease of non-specific immune response, there was also a significant decrease in phagocytic percent and phagocytic index for monocyte cells and the results in the first group were more significant than the second group .

While there were a non-significant decrease in polymorpho nuclear leucocyte P.M.N, for each test in exposed workers in general, compared with standard sample

The present study showed a significant increase in immuno globulin IgE in exposed workers compared to standard samples, and the elevation was more significant in the first group than in the second group .

The result of this study showed that significant decrease in total count of W.B.Cs in exposed workers compared with standard sample, significant decrease in total count of W.B.Cs in first group than second group .The differential count of W.B.Cs was showed a significant decrease in lymphocytes and monocyte while a significant increase  in esinophil was.detected .

This study showed a presence of positive interference for the following factors :  smoking, age , work site and work period with benzene exposure that lead to increase the poisoning effect of polycyclic aromatic hydrocarbons in petroleum exposed workers