دراسة التغيرات النسجية والكيموحيوية لعقار الليفاميزول في ذكور الفئران البيضاء

Study of Histological and Biochemical changes of Levamisole drug in white male mice

This study was conducted in July of 2008 in order to assess the effect of Levamisole drug immunological and toxicity effect chemically and Histologically with Immunoferone.

          Two doses of levamisole were given (3 and 10) mg/kg for body weight injected through Intrapretonial way. Two doses of Immunoferon (3 and 10) mg/kg for body weight were also given in the some way of the former one.

          Forty two mice were used in this study divided into six groups, seven mice of each as follow:

First group: control treatment (without any treatment)

Second group: 3 mg/kg for body weight of  Immunoferone.

Third group: 10 mg/kg for body weight of Immunoferone.

Fourth group: 3 mg/kg for body weight of  levamisole .

Fifth group: 10 mg/kg for body weight of  levamisole .

Sixth group: 50 mg/kg for body weight ofs  levamisole .

          Mice were weighed just before starting the treatment application, then after injection with drugs mentioned above. The weight of mice was record at weekly intervals. The experiment duration was (28) days. Blood samples from mice were taken from the heart after using anastatic from some parameters test required in this study. Results could be summarized as follow:

          Total protein was high in all treated groups of mice compared with the control group. Mice injected with levamisole drug gave higher total protein compared with those injected with immunoferone drug.

          Globulin was higher in all treated groups compared with the control treatment. Mice treated with levamisole gave higher globulin than those treated with Immunoferone. Blood serum glucose was significantly decreased in all treatments compared with the control. The reduction im blood serum in levamisole treatment was significant compared with the Immunoferone treatment. There was no significant influence due to treatment on the activity of GPT enzyme compared with the control treatment. Results of physiological tests record that, there was a significant increase in mice body weight compared with the control treatment. A significant increment was recorded from levamisole treatment compared with the Immunoferrone treatment as well as with control treatment.

          Histological test of internal organs gave immunological activities through the observation of higher number of lymphocyte cells in spleen of treated mice, extramedullary Haemoposis was also observed. Meanwhile histologically obvious effects were noticed in the animal giving a leathel does of drug leading to 100% death of mice treated with 50 mg/kg body weight of Levamisole represented by filitration of inflammatory cells (neutrophil), necrosis and congestion.

          Generally, it can be said, that levamisole drug effect was the better than Immunoferrone in terms of activation and increasing the body weight, through increasing the efficiency of immunological system compared with Immunoferrone .