دراسة تأثير المستخلصات الخام لنبات الصفصاف Salix acmophylla في نمو الخطوط الخلوية السرطانية والخلايا اللمفاوية الطبيعية للإنسان خارج الجسم

Study of the effect of crude extracts from Salix acmophylla on cancer cell lines and Human Normal Lymphocyte in vitro

This project was explorer to  study the activity of secondary metabolites in Salix acmophylla crude extracts on the malignant cells growth (in vitro) and study its effect on human lymphocytes .

This study included the preparation of fresh extracts for two main parts of this plant ( Bark and Leaves ) by using two types of solvents , distilled water and absolute ethanol . The results from leaves was 20% for L1 and 16% for L2 and from  bark is 6% for B1 and 4% for B2 . The active compounds of the extracts was  assessed  by using the chemical tests and the result showed  that acompound  contains  Glycosides , Tannins , Saponines and Flavonoides , but there is no Alkaloids , Terpenes and Steroids.

The action of these crude extracts of  S. acmophylla  was assessed on cancer cell lines ( HEP-2 AND AMN-3 ) and on normal cells of (REF) using seven concentrations ( 1000 , 500 , 250 , 125 , 62.5 , 31.25 , 15.6  µg / ml ) through three examination period ( 24 , 48 , and 72 hrs ) for cancer cell lines and 72 hrs only for normal cell line   of rat embryo (REF) with three exposure time.

The result is aclear cytotoxic activity of these crude extracts with high significances in a tow cancer cell lines during the three exposure time , suggesting that the cytotoxic effect of those crude extracts is a dose and time dependent , but in (REF) cell lines , there is no significant effect of those crude extracts reported.

The water extract was  found to be the most effective  especially on  the cell lines ( HEP-2 ) while on (AMN-3) cell lines the ethanol extract was more effective. It was non- significant differences were defected when  comparing the action of the leaves and the bark on these cell lines .

On the other hand  the inhibition  action  of these  Salix extracts were  examined on normal lymphocyte using the same seven concentration by counting the percentage of mitotic index and blastotic index in which L1,L2,B1,B2 show non significant   effect on  MI and BI in low concentration ,but show significant effect on high concentration  comparing with the control group.

Two experiments were conducted also to know if these extract have a mitogine , or have anti-mitogenic effects ,  thes extracts have no significant effect in both cases .