دراسة تأثير مستخلص اوراق القرنفل على بعض المتغيرات الفسيولوجية في ذكور الجرذ البيض المستحث بالكحول

Study effect of cloves leaf extract in some physiological changes in male albino rates induced by alcohol

Summary
My study was performed at college of Veterinary Medicine/University of Kerbala. It is performed during the period from 5 November of 2020 to 5 December 2020).The present study was designed to Investigate the antioxidant property of clove leaf extract on alcohol-induced liver damage via Determining the levels of enzymatic and non-enzymatic antioxidant (SOD ,GST ,Vit E ,Vit C ,GSH) ,and Assessment the hepato protective activity of Clove leaf extract to ameliorate The Physiological and Histological changes of alcoholic liver diseases via determining the liver function test (AST ,GGT ,unconjugated bilirubin , conjugated bilirubin) lipid levels (TG ,Cholesterol) and Histological changes in liver. forty males All experimental rats taken were weighing 250-300 gm .All rats were subdivided: (were divided into four subgroups according to the type of drug administered) for each group. 10 male rats were treated daily for thirty days . The first group: is the( control negative) administrated DMSO for 4 weeks . and the second group: will administrated clove leaf extract of 100 mg/kg body weight by intragastric oral. and the third group(control positive) : will administrated Ethanol 0.6g/kg body weight of 20% (v/v) by intragastric oral to induce liver alcoholic disease and The fourth group, will be administrated Ethanol + Clove leaf extract by Intragastric oral.
The results showed there was a significant elevation in the serum of AST, GGT, Conjugated bilirubin and Unconjugated bilirubin in (GIII) ethanol treated group and statistically significant increase in level of cholesterol and triglycerides ,in addition to decrease the serum of enzymatic and non-enzymatic antioxidants (SOD ,GST ,vitamin C, vitamin E and GSH in ethanol treated group (GIII) as compared with other groups .
After clove leaf extract administration we observe that there was a significant decrease (p>0.05) in serum AST , GGT ,Conjugated bilirubin ,Unconjugated bilirubin in (GIV) treated group and a significant decrease (p>0.05) in serum of cholesterol and triglycerides , in addition to increase the serum of enzymatic and non-enzymatic antioxidants (SOD ,GST ,vitamin C, vitamin E and GSH in (GIV) treated group in comparing to (GIII) group .
Histological changes in liver showed based on our data clove leaf extract ameliorates the physiological changes in alcoholic liver disease . Hepatocytes in ethanol-treated rats were irregular and enlarged, with mild fatty alterations such as fat droplets and fatty degeneration. In certain hepatocytes, degenerative changes and binucleation can be detected. Clove leaf-treated rats’ hepatocytes were more normal in structure, although they were multinucleated, indicating cellular regeneration. There were no symptoms of inflammation, necrosis, hemorrhage, or cholestasis in the clove leaf-treated rats. Clove leaf-treated rats’ livers were protected from ethanol-induced damage, according to these findings.
We conclude from this experiment that clove leaf extract has a very strong antioxidant activity because it contains phenolic substances that improve the condition of alcoholic liver disease.