دراسة فسلجية نسجية لتأثير المستخلص الكحولي لبذورنبات الحرمل Peganum harmala والكحول الأثيلي في ذكورالأرانب

Physiological –Histological Study To Effect Of Alcohol Extract Of Peganum Harmala Seeds And Ethanolic Alcohol In Male White Rabbit

summery
The present study was carried out in college of education for science
pure/university of kerbala,from october/2011 to june /2012.The study
involved fifty four healthy adult male rabbit white with red eyes Lepus
arcticus , the average weight (1,530-1,495) k.g t and (6-8) month old .The
rabbits were divided to the three groups , the first group was involved the
treatement of the alcoholic extract of the peganum harmala seeds,the second
group was involved the treated of the ethanol alcohol,the third group was
control treated with distteld water.The two groups were contains 18 rabbit
and divided in to three groups according to the different concentration to the
(10-20-30) mg/k.g,and contain for each the group on 6 rabbit for two
months.The third group was control treated with normal salin solution which
contain 18 rabbit for two months .The study was aimed to determine the
effect of peganum harmala extracts and ethanolic alcohol on some blood
parameters level of red blood cell,hemoglobin and platelate,and level of
enzymes AST, ALT and level of testosterone hormone,sperm count and level
of some proteins such as total protein,albumin,globuline.And study the
histological structure on the
liver,spleen,testis,epididymus,kidney,cerebral,cerebellum.The
subcatenous admenstration of peganum harmala seeds extracts physiologica
and histological results 1- significant
increase(p<0.05) in three concentration10, 20, 30 mg/k.g in levels of
concentration enzymes AST, ALT and in mean diameter of hepatocytes
&venous sinuses of red pulp in compared with control group.
2-significant increase (p<0.05) in10 mg/k.g in levels of testosterone
hormone,albumin ,and in mean diameter in germinal artery, seminiferous
tubules, spermatogonia, spermatozoa, epididymis tubule of ducts ,height
epithelial for head &tail of epididymis,glomerulus,distal convoluted tubule ,
inner granulous layer cells,and thickness in molecular layer in cerebral and
cerebellum ,outer granulous &pyramidal compared with control group .

3- significant increase (p<0.05) in 20 mg/k.g in mean diameter in central
veins,white pulp, spermatogonia, epididymis tubule of ducts , and in
thickness of multiform layer compared with control group
4- significant increase(p<0.05) in30 mg/k.g in mean diameter in sinusoids of
liver , central veins, germinal artery, white pulp& all layers in the cortex of
cerebral compared with control group.
5- significant decrease (p<0.05) in three concentration 10, 20, 30 mg/k.g in
levels of sperm count,total protein and in mean diameter in primary
spermatocytes, sertoli cells,proximal convoluted tubule, outer&inner cells in
pyramidal layers, cells in the multiformes layer and thickness of purkinje
layer& mean diameter of purkinje cells in three concentration compared
with control group.
6- significant dicrease(p<0.05) in10 mg/k.g in mean diameter in collecting
tubule ,height epithelial proximal convoluted tubule, cells in outer molecular
layer in cerebrallum, and thickness in inner granulous& pyramidal layers,
multiform layer, thickness inner granulous layer in cerebrallum compared
with control group
7- significant dicrease(p<0.05) in 20 mg/k.g in level testosterone
hormone,albumin, and thickness in molecular layer for cerebral, inner
granulous layer of cerebrallum compared with control group
8- significant dicrease(p<0.05) in 30 mg/k.g in level testosterone
hormone,albumin, platelate count,and in mean diameter in seminiferous
tubules, spermatozoa, collecting tubule,and thickness in inner granulous
layer of cerebrallum &the cells in the same layer compared with control
group .
The subcatenous admenstration of ethanol alcohol physiological and
histological results :-
1-significant increase(p<0.05) in three concentration 10, 20, 30 mg/k.g in
levels of total protein, globuline and in mean diameter in hepatocytes, height
epithelial of distal convoluted tubule, cells in outer molecular layer for
cerebrallum and thickness in outer granulous &pyramidal layers in
compared with control group.
2- significant increase (p<0.05) in 10 mg/k.g in levels of albumin and in
mean diameter in germinal artery, spermatogonia, spermatozoa, cells in inner
granulous layer of cerebrallum compared with control group.
3- significant increase (p<0.05) in 20 mg/k.g in levels of albumin, AST,ALT
and in mean diameter in spermatogonia ,seminiferous tubules , glomerulus
,and thickness in purkinje layer compared with control group.
4- significant increase(p<0.05) in 30 mg/k.g in levels enzymes AST,ALT,
and in mean diameter in glomerulus, central veins,white pulp, venous sinuses
of red pulp, epididymis tubule of ducts , proximal convoluted tubule,
collecting tubule,cells in inner granulous layer of cerebrallum and thickness
in purkinje layer ,outer molecular layer for cerebrallum and all layers in the
cortex of cerebral compared with control group.

5- significant dicrease(p<0.05) in levels of sperm count, and in mean
diameter in purkinji cells,sertoli cells in three concentration 10, 20, 30
mg/k.g compared with contro lgroup .

6- significant dicrease(p<0.05) in 10 mg/k.g in mean diameter in
seminiferous tubules, epididymis tubule of ducts,height epithelial for
head&tail of epididymis, glomerulus, proximal convoluted tubule, collecting
tubule, and thickness in multiform layer, molecular layer in cerebral, inner
granulous &pyramidal layers ,purkinje layer, inner granulous layer for
cerebrallum compared with control group.
7- significant dicrease(p<0.05) in 20 mg/k.g in level of red blood cell
count,hemoglobin ,platelate, in mean diameter in venous sinuses of red pulp,
cells in the molecular layer of cerebral and thickness in multiform layer and
the cells in the same layer compared with control group.
8- significant dicrease(p<0.05) in 30mg/k.g in level testosterone hormone,
red blood cell count, hemoglobin ,platelate, in mean diameter in germinal
artery, seminiferous tubules,cells in molecular layer in cerebral compared
with control group.