دور بروتين الصدمة الحرارية – 70 (HSP-70) والبروتينات المرتبطة بفيتامين D3 (VDBP) وعلاقته بجينات مستقبلات فيتامين D3 بين النساء البدينات المصابات بداء السكر النوع الثاني

Role of heat shock protein 70 and vitamin D3-binding protein and its relation to vitamin D3 receptor genes in obese women with type 2 diabetes

The current study included 45 woman with type 2 diabetes mellitus attend to Al_Hussein Teaching Hospital and Al_Hassan center of diabetes and endocrinology unit / kerbala health Directorate_lraq,their ages ranged between (30_67) year, and they were divided into two groups. Depending on the body mass index (BMI), it included the group of obese women with an average body mass of more than 30 kg/m2, and the group of normal weight women with average body mass of less than 30 kg/m2, the sampling period lasted from the beginning of April (2022) to March (2023). As well as 45 healthy women with out any health problem and at the same ages as a control group proteins.
The results observed indicated that HSP-70 and VDBP levels shown a non-significant decrease in all of type 2 diabetic groups as compared with apparently healthy control group, while the C- peptide were significantly decreased in type 2 diabetic patients when compared with apparently control group. In addition, the random blood glucose and HbA1c% were shown significant elevation in type
2 diabetic patients as compared with apparently healthy control groups. The observed data indicated that HSP70 , VDBP and C- peptide levels when comparing between type 2 diabetic patients and control in obese groups shown a risk factors depending upon the odd ratio observed (OR = 1.156(1.649-1.148), 0.917(0.733-1.148)and 1.049(1.011-1.088)) respectively, but only C-peptide was shown to be significant. In BMI the levels of HSP- 70, VDBP and C-peptide, as shown the HSP70, VDBP and C- peptide was non-significant in obese groups, while the HSP-70VDBP and C-peptide was shown significant in normal weight groups. The optimal diagnostic points for HSP70 was (sensitivity =90%, specificity = 87%) at a level (Cut- off points) = 29.086, while C-Peptide levels: (sensitivity = 93%, specificity = 88%) at a level (Cut-off points) = 134.76, and VDBP (sensitivity = 96%, specificity
= 78%) at a level (Cut-off points) = 56.423 all markers have p- values of the AUC were <0.001 and statistically significant.
Accordingly, it was concluded that a significant relationship between circulating VDBP levels and T2DM. VDBP appears to able to contribute to the treatment of obesity and diabetes because of its anorexigenic and antihyperglycemic effects. In addition, C- peptide is a known biomarker of insulin resistance and beta-cell function. High specificity and sensitivity analyzed results were obtained by ROC analysis for both markers in T2DM.And the result of genotype, In addition, When compared VDR with polymerase gene in patients showed increased frequencies of HSP-70: CT + TT and TT genotypes in (rs1544410) for CT (OR = 0.972(0.901 – 1.048), p = 0.464; TT (OR = 0.988(0.921 – 1.059), p = 0.734, respectively), VDBP: CT + TT and TT genotypes in (rs1544410) for CT (OR = 0.998(0.969 – 1.027), p = 0.875; TT (OR = 0.988(0.961 – 1.015), p = 0.384,. C-Peptide : CT + TT and TT genotypes in (rs1544410) for CT (OR = 1.003(0.997 – 1.009), p = 0.338; TT (OR 0.998(0.990 – 1.006), p = 0.593, Just significant differences in 25(OH)D3