دور حمض ألفا ليبويك في بعض المعاير الكيميو حيويه والسلوكية في الضرر العصبي المستحدث بواسطة د- جالاكتوز في ذكور الجرذان

Role of alpha lipoic acid on some biochemical parameters and behaviorals of D-galactose induced neurological damage in male rats

This study is carried out to investigate the protective role of alpha lipoic acid (ALA) on the central nerves system damage induced by d-galactose overload in male rats.
Forty adult male rats were divided into four groups (10 / group), the first group was injected with normal Saline for thirty (30) days and served as control group (control). Rats in the second group were injected with 200 mg/kg B .W IP for 30 days of (D-gal), rats of the third group (ALA) were injected with (D-gal) (IP) with 100 mg/kg B .W for 30 days, and the fourth group (D-gal+ALA) was injected D-gal 200 mg/kg B.W with ALA 100 mg/kg B.W. daily for 30 days. The experiment was carried out in the laboratories of the college of veterinary medicine, university of karbala.
Fasting blood samples were collected by cardiac punctures technique at thirty (30) days of experiments for collecting the brain tissue for measuring: beta amyloid, glutamate, serum acetylcholine Esterase(AChE) activity ,total anti-oxidant capaci-ty(TAC), Malondialdehyde (MDA), reduced Glutathione(GSH) concentration, cata-lase (CAT), superoxide dismutase (SOD) activities and assessment of behavioral anal-ysis forced swimming test (FST),light/dark test(LDT) and memory tests morris water maze Test(MWM),elevated plus-maze test (EPM).
The results showed a significant increase (P≤0.05) in the brain tissue beta amy-loid value in D-gal treated group as compared to control, ALA and (D-gal+ALA) groups. There was a significant decrease (P≤0.05) in the tissue glutamate concentration in ALA treated group when compared to control, ALA and (D-gal+ALA). While, there were no significant (P≥0.05) changes between control, ALA and (D-gal+ALA) groups. In addition, the result showed a significant decrease in the serum (TAC) con-centration (P≤0.05) in the d-gal treated group when compared to control , ALA and (D-gal+ALA), While there was no significant (P≥0.05) changes between control , ALA and (D-gal+ALA) groups. serum GSH concentration shows a significant de-crease (P≤0.05) in D-gal group when compared with control, ALA and (D-gal+ALA) groups.There was no significant (P≥0.05) between control, ALA and (D-gal+ALA) groups.
The current study showed a significant increase MDA concentration (P≤0.05) in D-gal group when compared with control, ALA and (D-gal+ALA) groups.There was no significant (P≥0.05) D-gal change between control, ALA and (D-gal+ALA) groups, While, serum (CAT) activity showed a significant decrease (P≤0.05) D-gal when compared with control, ALA and (D-gal+ALA) groups. There was no signifi-cant ALA group (P≥0.05) change between control , ALA and (D-gal+ALA) groups .serum (SOD) concentration showed a significant decrease (P≤0.05) in D-gal group when compared with control , ALA and (D-gal+ALA) groups.There were no signifi-cant (P≥0.0001) d-gal changes between control, ALA and (D-gal+ALA).
Behavioral analysis and memory test showed a significant decrease in the FST (p≤0.05) in D-gal group when compared with control, ALA and (D-gal+ALA) groups.There was no significant (p≥0.05) D-gal between control, ALA and (D-gal+ALA) groups. While showed a significant (p≤0.05) increase in the (MWM) in D-gal group when compared to control, ALA and (D-gal+ALA), and there were no sig-nificant (p≥0.05) changes in the D-gal between control, ALA and (D-gal+ALA) groups.light room test showed a significant increase (P≤0.05) in D-gal treated group when compared to control, ALA and (D-gal+ALA), also there was no significant (P≥0.05) d-gal change between control, ALA and (D-gal+ALA) groups.Dark Room test shows a significant decrease (P≤0.05) in d-gal treated group when compared to control, ALA and (D-gal+ALA), also there were no significant (P≥0.05) D-gal changes between control, ALA and (D-gal+ALA) groups.
Elevated plus-maze test \ open arm showed a significant increase (P≤0.05) af-ter 30 days in D-gal treated group when compared to control, ALA and (D-gal+ALA), also there were no significant (P≥0.05) D-gal changes between control, ALA and (D-gal+ALA) groups. Elevated plus-maze test \ closed arm showed a significant decrease (P≤0.0001) in D-gal treated group when compared to control, ALA and (D-gal+ALA), also there were no significant (P≥0.05) D-gal changes between control, ALA and (D-gal+ALA) groups.

In conclusion, our results revealed that ameliorated role of the ALA on the ox-idative stress and deterioration of the behavior and memory in the male rats was in-duced by D-gal.