علاقة تعدد اشكال جينات بمتلازمة تكيس المبايض TLR4 و CD14

Association of TLR4 and CD14 genes polymorphism with polycystic ovarian syndrome

Abstract

Polycystic ovarian syndrome (PCOS), is a common inflammatory endocrine disorder that affects women of reproductive age and considered risk factor for infertility. polymorphism is associated with an increased risk of PCOS.
The study aimed to investigate the association between PCOS and genetic polymorphism of the Toll Like Recerptor 4 (TLR4) gene rs4986790 as well as the Cluster differentiation 14(CD14) gene rs2569190 involved in innate immunity in Iraqi women with this syndrome
The subjects of the study are divided into two groups controls(n=45) and patients(n= 45) enrolled in the AL-Sader Teaching Medical City and AL-Zahraa Teaching Hospital during the period from August 2020 to January 2021. The diagnosis of subjects was according to the Rotterdam Consensus (2003).
Deoxyribonucleic acid extracted from peripheral blood samples and genotyping done for CD14 and TLR4 genes. Genotyping of TLR4 (299A>G) and CD14 −159C>T polymorphisms was performed using the tetra-primer amplification refractory mutation system– polymerase chain reaction. The TNF- alpha was done by serological technique ELISA.
The result of the study revealed that TLR4 (allele and genotype), CD14 (allele and genotype) and TNF-α level were significant differance (P<0.05). There was a significant difference at (P<0.05) in the comparing between control and the patient TNF-α in TLR4 and CD14. Infertility was significantly related to CD14 over-dominant groups of CC-CT with CT (P=0.022), Whereas it was not significant to the other genotype groups (P>0.05). There was no significant difference for TNF- α according to infertility (P=0.611). the current study shows that patients with Polycystic ovarian syndrome have single nucleotide polymorphism (SNPs) in TLR4 (rs4986790) and in CD14 (rs2569190) and levels of tumor necrosis factor-a (TNF-a) are higher.