تأثير خلات الرصاص في بعض المعايير الفسيولوجية والوراثية في ذكور الجرذان البيض

Effect of Lead Acetate in Some Physiological & Genetic Parameters in White Male Rat Rattus rattus

This study aimed to investigate the effect of  lead on total body weight of  animals and some somatic organs and male reproductive organs and some accessory glands and effect on levels of  Luteinizing hormone and testosterone hormone & levels of total protein and parameter of  sperm (sperm count ,percent of motility , viability, normality of sperm) and effect on diameter & thick of germ cell layer and percent of damage in seminiferous tubules and diameter& thick of epithelial layer of epididymis and effect of mitotic index. The experiment were conducted on (40) adult male of  rat which were randomly divided into two main groups depending on length of gavage  to(35&70 day).Each main group was subdivided to three sub groups depending on the concentration of lead to(8,16,24 mg/kg) in addition to control group which gavage with normal saline. Body weight was calculated before and after each experiments. The animals were killed after 24 hours from the last dose of treatment. The blood samples were collected. The levels of hormones and total protein were measured. Liver ,kidney ,spleen ,testes ,epididymis ,prostate ,seminal vesicles was removed, weighted, processed for histological study. for studying the effecting of lead on percent of  damage and the changeables of mean diameters thick of germ cell layer of seminiferous tubules and changeable in mean diameters thick of epithelial layer of epididymis were compared with control groups. Femur was removed to account of mitotic index. The results reveal some significant differences when comparision  were made between the experimental groups and the control as follow:

      A significant decrease (P<0.05) in animals weight and level of total protein treated for all dose of  gavage to(35 &70day)and  significant decrease (P<0.05) in spleen weight  treated for all dose of  gavage to (35 &70day) except the group treatment with dose in concentration (8 mg/kg) for (35 &70day). and significant decrease (P<0.05) in weight of liver , kidney ,testes, epididymis , prostate ,seminal vesicle and sperm count ,percent of motility , viability, normality of sperm and diameter of seminiferous tubules and diameter& thick of epithelial layer of epididymis treated  and significant increase(P<0.05) in percent of damage in seminiferous tubules treated for all dose of gavage to(35 &70day) .except the group treatment with dose in concentration (8 mg/kg) for 35 day. and significant decrease (P<0.05) in levels of Luteinizing hormone and testosterone hormone treated for all dose of  gavage to (35 &70day) except the group treatment with dose in concentration (8 mg/kg) for 35 day.and significant increase(P<0.05) in mitotic index treated for all dose of  gavage to (35 &70day) .

        Different changeable of histopatholgical were found in testes and epididymis in addition found chromosomal aberration  of  anomals depend on doses and period of treatment of lead acetate .