إضافة حمض الفوليك إلى النظام الغذائي لدجاج من نوع لوهمان لتحسين الصفات النوعية للبيض والحالة الصحية

Supplementation of Folic Acid to Lohmann hens Dite to Optimize Eggs Quality and Health Status

This study was carried out to investigate the effect of supplementation of folic acid enriched diet on folic acid concentration of egg , serum , egg quality, immune response and intestinal morphology of laying hens .Five thousand lohmann classic brown laying hen’s type were use in this experiment.
At the first period hens were fed on basal diet according to Lohmann Classic Brown nutrition requirement guide. It was contain a calculated amount from folic acid. This amount was coming from row diet premix. The Second period of the experiment all hens were fed same basal diet enriched with folic acid with 1mg/kg of feed.
Egg was collected and blood samples were collected from the Lehman’s at the end of the first and second periods for measuring folic acid concentration for egg and serum by using HPLC and ELISA. Also immune response were tested by using ELISA. Egg quality were measuring by calculating of egg weight, albumin height, albumin diameter, yolk height, yolk diameter, yolk color, shell thickness and Haugh unit.
Duodenum, jejunum and ileum intestine sample were collected at the end of the first and second period for measuring villi height and crypt depth.
The results showed a significant increase of folic acid concentration of serum and egg after supplementation of 1 mg /kg feed of folic acid. ELISA serum folic acid concentration was recorded 145.4 ng/mL after supplementation of folic acid as Compare before supplementation with folic acid was recorded 79 ng/mL. On the other hand HPLC folic acid concentration after supplementation of folic acid to the diet was recorded 64.9 ppm as compare before supplementation with folic acid that was recorded 32 ppm. Egg quality was enhance after supplementation of folic acid. Egg weight, albumin height diameter, yolk height, yolk diameter, yolk color and Haugh unit were enhanced significantly (p≤0.05) after supplementation with folic acid, and egg shall thickness show no significant change between the two period.
Villi height and crept depth showed a significant difference (p≤0.05) in second periods after supplementation of folic acid compare to first period before folic supplementation. The highest enhancement of villi hight and crypt depth was observed in duodenum and jejunum. However, Villi height and crept depth illium show no significant deference between the two periods.
The highest folic egg concentration will produce folic acid egg called egg folate this will help pregnant woman to reduce spinal cord defect in fetus. A rich laying hens diet may be enhance of egg quality, immune response to hens that consumes this diet. Gut health state may enhanced consuming of diet enrich with folic acid. Enhancing of villi hight and crypt depth lead to enhance of gut health state. This enhancement may be consuming of diet rich with folic acid.